脂质体大小对增强包封的三亚乙基四胺六乙酸（TTHA）抗镉中毒功效的影响。

Influence of size of liposomes in potentiating the efficacy of encapsulated triethylenetetramine-hexaacetic acid (TTHA) against cadmium intoxication.

作者信息

Behari J R, Gupta S, Srivastava S, Srivastava R C

出版信息

Ind Health. 1993;31(1):29-33. doi: 10.2486/indhealth.31.29.

DOI:10.2486/indhealth.31.29

Abstract

Polyaminocarboxylic acids have widely been used an antidotes in heavy metal intoxication, however their hydrophilic nature renders them to be mostly distributed extracellularly. To facilitate the intracellular delivery of such chelating agent, triethlenetetraamine-hexaacetic acid (TTHA) was encapsulated in small unilamellar vesicles (SUV) or dehydration rehydration vesicles (DRV) and its effect was examined in the amelioration of cadmium toxicity. Mice were administered cadmium (0.2 mg/kg B.wt.) as CdCl2 intraperitoneally daily for five days. After a period of four weeks rest. they were given two intravenous injections of TTHA as free material or encapsulated in liposomes (0.16 m mole/kg) at a gap of 48 hours. Urinary and fecal elimination of cadmium and its distribution in the liver, kidney and spleen was monitored after TTHA treatment. The results indicate the efficacy of TTHA in removing cadmium from the body organs of preexposed animals and its excretion through urine and feces was maximum when it was encapsulated in SUV liposomes.

摘要

聚氨基羧酸已被广泛用作重金属中毒的解毒剂，然而其亲水性使其大多分布于细胞外。为促进此类螯合剂的细胞内递送，将三亚乙基四胺六乙酸（TTHA）包裹于小单层囊泡（SUV）或脱水再水化囊泡（DRV）中，并检测其对镉毒性的改善作用。小鼠每日腹腔注射氯化镉（0.2 mg/kg体重），持续五天。休息四周后，它们在48小时间隔内接受两次静脉注射，分别为游离形式的TTHA或包裹于脂质体中的TTHA（0.16 mmol/kg）。在TTHA治疗后监测镉的尿排泄和粪排泄及其在肝脏、肾脏和脾脏中的分布。结果表明，TTHA在从预先暴露动物的身体器官中去除镉方面具有功效，当它被包裹于SUV脂质体中时，其通过尿液和粪便的排泄量最大。