Li Zhaopeng, Zhang Xu, Tan Tianwei
College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, PR China.
Biotechnol Lett. 2006 Apr;28(7):477-83. doi: 10.1007/s10529-006-0002-y.
Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose after batch culture, thereby giving hsBLyS at 3.7 g l(-1) and a productivity of 0.11 g l(-1) h(-1).
在大肠杆菌的补料分批培养中，采用四种不同的补料分批培养策略，以乳糖替代异丙基-β-D-硫代半乳糖苷（IPTG）作为诱导剂，进行人可溶性B淋巴细胞刺激因子（hsBLyS）的过量表达。由于乳糖既是诱导剂又是碳源，最佳且最简单的培养策略是在分批培养后直接补加乳糖，从而获得了3.7 g l⁻¹的hsBLyS，生产率为0.11 g l⁻¹ h⁻¹。
