Johnson R, Masserano R, Haring R, Kho B, Schilling G
J Pharm Sci. 1975 Jun;64(6):1007-11. doi: 10.1002/jps.2600640626.
A GLC method is described for the quantitative analysis of conjugated estrogens. The procedure was used for the analysis of various estrogen mixtures obtained from formulations containing estrogen sodium sulfate salts. The method involves slurrying or dissolving a portion of the formulation pH 5.2 buffer, hydrolysis with sulfatase enzyme, extraction with ethylene dichloride, anf finally, GLC separation and quantitation of the corresponding trimethylsilyl ethers on a 2.5% diethylene glycol succinate column.
描述了一种用于定量分析结合雌激素的气相色谱法。该程序用于分析从含有硫酸雌激素钠盐的制剂中获得的各种雌激素混合物。该方法包括将一部分制剂在pH 5.2缓冲液中制成浆液或溶解，用硫酸酯酶水解，用二氯乙烷萃取，最后在2.5%丁二酸二乙二醇酯柱上进行气相色谱分离和定量相应的三甲基硅醚。
