Modine T, Azzaoui R, Fayad G, Lacroix D, Bordet R, Warembourg H, Gourlay T
Service de Chirurgie Cardiovasculaire, Hôpital Cardiologique, CHRU Lille, France.
Perfusion. 2006 Mar;21(2):87-92. doi: 10.1191/0267659106pf854oa.
This study was undertaken to develop a rodent (rat) model of cardiopulmonary bypass (CPB) that has been designed to mimic functionally the minimally invasive clinical setting. The circuit is similar to the clinical model in terms of its construction, configuration, material surface area to blood volume ratio, and priming volume to blood ratio. The overall priming volume was 10 mL. Thirty-six male Sprague-Dawley rats (422 +/- 32 g) were anaesthetized while maintaining spontaneous ventilation. Anticoagulation was achieved with heparin (500 IU/kg). Blood arterial pressure was monitored continuously. Normal central temperature was maintained throughout. Intermittent arterial blood gas levels also were monitored. All animals were cannulated in preparation for CPB; however, CPB, utilizing a double roller pump and a flow rate of 100 mL/kg/minute for 60 min, was initiated in only 18 animals, the remaining 18 animals acting as non-CPB controls (Sham). The animals were haemodynamically stable. After the operative procedure, the animals were allowed to recover from the anaesthesia and, after transfer to a recovery facility, were monitored for a period of 1 week. There were no differences between the groups in terms of blood gas analysis and blood pressure data; all animals survived the procedure and had an uneventful follow-up. Differences were found between the CPB animals and the Sham group in terms of TNFalpha used as a marker of inflammatory processes. This trend tends to support this model as an analogue for the clinical scenario for future studies of CPB-related inflammation. Overall, the CPB procedure was easy to perform and was associated with excellent survival. This recovery model is an effective tool to perform pathophysiological studies associated with minimally invasive CPB.
本研究旨在建立一种体外循环（CPB）啮齿动物（大鼠）模型，该模型在功能上模拟微创临床环境。该循环系统在结构、配置、材料表面积与血容量比以及预充液体积与血液比方面与临床模型相似。总预充液体积为10 mL。36只雄性Sprague-Dawley大鼠（422±32 g）在维持自主通气的情况下进行麻醉。用肝素（500 IU/kg）实现抗凝。持续监测动脉血压。全程维持正常中心体温。还间歇性监测动脉血气水平。所有动物均进行插管以备CPB；然而，仅18只动物开始进行CPB，使用双滚轴泵，流速为100 mL/kg/分钟，持续60分钟，其余18只动物作为非CPB对照组（假手术组）。动物血流动力学稳定。手术后，让动物从麻醉中恢复，转移到恢复设施后，监测1周。两组在血气分析和血压数据方面无差异；所有动物手术存活且随访顺利。在用作炎症过程标志物的TNFα方面，CPB动物与假手术组之间存在差异。这一趋势倾向于支持该模型可作为未来CPB相关炎症研究临床场景的类似物。总体而言，CPB操作简便，存活率高。这种恢复模型是进行与微创CPB相关病理生理学研究的有效工具。
