Shepard Jason R E
Wadsworth Center, New York State Department of Health, Albany, New York 12208, USA.
Anal Chem. 2006 Apr 15;78(8):2478-86. doi: 10.1021/ac060011w.
High-throughput microscale platforms have transformed modern analytical investigations. Traditional microarray analyses involve a comparative approach, with two samples, a known control and an unknown sample, hybridized side-by-side and then contrasted for genetic differences. The samples are labeled with separate dyes and hybridized together, providing a differential expression pattern based on the reporter intensities. In contrast, the fiber-optic microarray platform described herein is analyzed with a microscope, thereby enabling the use of virtually any reporter, including quantum dots. The instrumentation takes advantage of the narrow emission bands characteristic of quantum dots to perform multiplexed detection of Bacillus anthracis. Advancing beyond the standard red/green microarray experiment, a panel of eight reporters were linked to eight B. anthracis samples and simultaneously analyzed in a microarray format. The ability to employ an assortment of reporters, along with the capacity to simultaneously hybridize eight samples confers an unprecedented flexibility to array-based analyses, providing a 4-fold increase in throughput over standard two-color assays.
高通量微尺度平台已经改变了现代分析研究。传统的微阵列分析采用比较方法，将两个样本，即一个已知对照和一个未知样本，并排杂交，然后对比基因差异。样本用不同的染料标记后一起杂交，根据报告分子强度提供差异表达模式。相比之下，本文所述的光纤微阵列平台通过显微镜进行分析，从而能够使用几乎任何报告分子，包括量子点。该仪器利用量子点特有的窄发射带对炭疽芽孢杆菌进行多重检测。超越标准的红/绿微阵列实验，一组八个报告分子与八个炭疽芽孢杆菌样本相连，并以微阵列形式同时进行分析。使用各种报告分子的能力，以及同时杂交八个样本的能力，赋予了基于阵列分析前所未有的灵活性，与标准双色检测相比，通量提高了4倍。
