通过蛋白质相关性分析绘制的哺乳动物细胞器图谱。

A mammalian organelle map by protein correlation profiling.

作者信息

Foster Leonard J, de Hoog Carmen L, Zhang Yanling, Zhang Yong, Xie Xiaohui, Mootha Vamsi K, Mann Matthias

机构信息

Center for Experimental BioInformatics (CEBI), Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

出版信息

Cell. 2006 Apr 7;125(1):187-99. doi: 10.1016/j.cell.2006.03.022.

DOI:10.1016/j.cell.2006.03.022

PMID:16615899

Abstract

Protein localization to membrane-enclosed organelles is a central feature of cellular organization. Using protein correlation profiling, we have mapped 1,404 proteins to ten subcellular locations in mouse liver, and these correspond with enzymatic assays, marker protein profiles, and confocal microscopy. These localizations allowed assessment of the specificity in published organellar proteomic inventories and demonstrate multiple locations for 39% of all organellar proteins. Integration of proteomic and genomic data enabled us to identify networks of coexpressed genes, cis-regulatory motifs, and putative transcriptional regulators involved in organelle biogenesis. Our analysis ties biochemistry, cell biology, and genomics into a common framework for organelle analysis.

摘要

蛋白质定位于膜包被的细胞器是细胞组织的核心特征。利用蛋白质相关性分析，我们已将1404种蛋白质定位到小鼠肝脏的十个亚细胞位置，这些位置与酶活性测定、标记蛋白谱以及共聚焦显微镜观察结果相符。这些定位有助于评估已发表的细胞器蛋白质组清单中的特异性，并表明39%的细胞器蛋白存在多个定位。蛋白质组学和基因组数据的整合使我们能够识别参与细胞器生物发生的共表达基因网络、顺式调控基序以及推定的转录调节因子。我们的分析将生物化学、细胞生物学和基因组学联系到一个用于细胞器分析的通用框架中。