揭示相位问题的紫外线。

Shedding UV light on the phase problem.

作者信息

Sussman Joel L, Silman Israel

出版信息

Structure. 2006 Apr;14(4):629-30. doi: 10.1016/j.str.2006.03.004.

DOI:10.1016/j.str.2006.03.004

Abstract

In this issue of Structure, Nanao and Ravelli (2006) describe the use of UV-induced radiation damage (UV-RIP) to solve the phase problem for proteins, employing single-wavelength X-ray radiation, without the need for derivatization. This should also permit data collection for many proteins on home sources, without travel to a synchrotron.

摘要

在本期《结构》杂志中，七尾和拉韦利（2006年）描述了利用紫外线诱导的辐射损伤（UV-RIP）来解决蛋白质的相位问题，采用单波长X射线辐射，无需进行衍生化处理。这也应该能够使许多蛋白质在实验室光源上进行数据收集，而无需前往同步加速器。