Lin Yi, Zeng Yaoying, Zeng Shan, Wang Tong
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou City, China.
Fertil Steril. 2006 Apr;85 Suppl 1:1125-9. doi: 10.1016/j.fertnstert.2005.08.056.
To investigate the potential role that toll-like receptor 3 (TLR3) may play in a murine model of polyinosinic-polycytidylic acid (polyIC)-induced embryo resorption.
An animal model-based study on induced embryo resorption.
University animal laboratory.
ANIMAL(S): Pathogen-free animals housed under barrier conditions and monitored for health status. Pregnant BALB/c mouse mated by C57BL/6 male was used as a model of allogeneic gestation.
INTERVENTION(S): The administration of polyIC was performed to establish a murine model of induced embryo resorption, with or without TLR3 blocking by multiple injection of mAb against this receptor.
MAIN OUTCOME MEASURE(S): Flow cytometric analysis was performed to detect the percentage of CD45(+)DX5+ and DX5(+)CD69+ cell subsets, with the DX5 antigen used as a common natural killer (NK) cell marker, and CD69 as a marker for activated NK cells, respectively.
RESULT(S): In the allogeneic mating model, BALB/cxC57BL/6, both the CD45(+)DX5+ and DX5(+)CD69+ cell percentages were significantly elevated upon polyIC stimulation at the absence of anti-TLR3 administration but were kept unchanged if the female mice were pretreated with anti-TLR3 monoclonal antibody. Accordingly, the resorption rate of embryos was boosted by polyIC administration, but this effect could be abrogated by pretreatment of anti-TLR3 mAb.
CONCLUSION(S): The engagement of polyIC with TLR3 may be critical to the activation of NK cells infiltrated at the feto-maternal interface, subsequently resulting in an increase in embryo resorption.
研究Toll样受体3（TLR3）在聚肌苷酸-聚胞苷酸（polyIC）诱导的小鼠胚胎吸收模型中可能发挥的潜在作用。
基于动物模型的诱导胚胎吸收研究。
大学动物实验室。
在屏障条件下饲养并监测健康状况的无病原体动物。将与C57BL/6雄性小鼠交配的怀孕BALB/c小鼠用作同种异体妊娠模型。
给予polyIC以建立诱导胚胎吸收的小鼠模型，通过多次注射抗该受体的单克隆抗体（mAb）进行或不进行TLR3阻断。
进行流式细胞术分析以检测CD45(+)DX5+和DX5(+)CD69+细胞亚群的百分比，DX5抗原分别用作常见的自然杀伤（NK）细胞标志物，CD69用作活化NK细胞的标志物。
在同种异体交配模型BALB/cxC57BL/6中，在未给予抗TLR3的情况下，polyIC刺激后CD45(+)DX5+和DX5(+)CD69+细胞百分比均显著升高，但如果雌性小鼠用抗TLR3单克隆抗体预处理，则保持不变。因此，polyIC给药可提高胚胎吸收速率，但抗TLR3 mAb预处理可消除这种作用。
polyIC与TLR3的结合可能对激活浸润于母胎界面的NK细胞至关重要，随后导致胚胎吸收增加。
