拟南芥高亲和力己糖转运蛋白STP13的表达与程序性细胞死亡相关。
Expression of the Arabidopsis high-affinity hexose transporter STP13 correlates with programmed cell death.
作者信息
Norholm Morten H H, Nour-Eldin Hussam H, Brodersen Peter, Mundy John, Halkier Barbara A
机构信息
Plant Biochemistry Laboratory, center for Molecular Plant Physiology, the royal Veterinary and Agricultural University, Fredericksberg C, Denmark.
出版信息
FEBS Lett. 2006 Apr 17;580(9):2381-7. doi: 10.1016/j.febslet.2006.03.064.
We report the biochemical characterization in Xenopus oocytes of the Arabidopsis thaliana membrane protein, STP13, as a high affinity, hexose-specific H(+)-symporter. Studies with kinase activators suggest that it is negatively regulated by phosphorylation. STP13 promoter GFP reporter lines show GFP expression only in the vascular tissue in emerging petals under non-stressed conditions. Quantitative PCR and the pSTP13-GFP plants show induction of STP13 in programmed cell death (PCD) obtained by treatments with the fungal toxin fumonisin B1 and the pathogen Pseudomonas syringae. A role for STP13 in PCD is supported by microarray data from e.g. plants undergoing senescence and a strong correlation between STP13 transcripts and the PCD phenotype in different accelerated cell death (acd11) mutants.
我们报道了拟南芥膜蛋白STP13在非洲爪蟾卵母细胞中的生化特性，它是一种高亲和力、己糖特异性的H⁺同向转运体。用激酶激活剂进行的研究表明，它受到磷酸化的负调控。STP13启动子绿色荧光蛋白报告株系显示，在非胁迫条件下，绿色荧光蛋白仅在正在发育的花瓣的维管组织中表达。定量PCR和pSTP13-GFP植物显示，在用真菌毒素伏马菌素B1和病原菌丁香假单胞菌处理诱导的程序性细胞死亡（PCD）中，STP13被诱导表达。例如，来自衰老植物的微阵列数据以及不同加速细胞死亡（acd11）突变体中STP13转录本与PCD表型之间的强相关性，支持了STP13在PCD中的作用。
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