Lindeboom J A, Mathura K R, Ramsoekh D, Harkisoen S, Aartman I H, van den Akker H P, Ince C
Department of Physiology, Academic Medical Center Amsterdam and Academic Center for Dentistry Amsterdam (ACTA), The Netherlands.
Arch Oral Biol. 2006 Aug;51(8):697-702. doi: 10.1016/j.archoralbio.2006.02.009. Epub 2006 Apr 17.
In this study we evaluated the inter-observer agreement in the assessment of gingival capillary density using Orthogonal Polarization Spectral Imaging.
In this study gingival capillary density of 100 healthy subjects was determined by 2 independent observers. Agreement was quantified by calculation of the mean differences between the observers and the standard deviation of this difference and the limits of agreement. Reliability was quantified by means of the intraclass correlation coefficient (ICC).
Fifty males and 50 females were included in the study. The mean age for males was 20+/-1.2 years and for females 20+/-1.4. OPS images showed remarkable good quality images of the gingival microcirculation. The interclass correlation between the 2 observers was 0.63 while the interclass correlation for the 6 measurements in observer 1 was 0.95 and 0.94 for observer 2. The mean capillary density for females in observer 1 was 83.69+/-16.4 and 83+/-16.0 in observer 2, versus 60.55+/-12.3 for observer 1 and 60.4+/-12.1 for males. The mean quantitative functional capillary density in male students was 60.48+/-10.7, compared to 83.45+/-13.5 in female students.
OPS imaging enabled for the first time direct in vivo visualization and quantification of human functional gingival capillary density in healthy medical students. The inter-observer agreement was found to be good to fair on the quantification of gingival capillary density between the two independent observers. The intracorrelation coefficient (0.95) was excellent when assessing the reliability of one observer.
在本研究中，我们评估了使用正交偏振光谱成像技术评估牙龈毛细血管密度时观察者间的一致性。
在本研究中，100名健康受试者的牙龈毛细血管密度由2名独立观察者测定。通过计算观察者之间的平均差异、该差异的标准差以及一致性界限来量化一致性。通过组内相关系数（ICC）来量化可靠性。
该研究纳入了50名男性和50名女性。男性的平均年龄为20±1.2岁，女性为20±1.4岁。正交偏振光谱成像显示了质量极佳的牙龈微循环图像。两名观察者之间的组间相关性为0.63，而观察者1的6次测量的组间相关性为0.95，观察者2为0.94。观察者1中女性的平均毛细血管密度为83.69±16.4，观察者2中为83±16.0，而观察者1中男性为60.55±12.3，观察者2中为60.4±12.1。男学生的平均定量功能性毛细血管密度为60.48±10.7，女学生为83.45±13.5。
正交偏振光谱成像首次实现了对健康医学生牙龈功能性毛细血管密度的直接体内可视化和量化。两名独立观察者在牙龈毛细血管密度量化方面的观察者间一致性良好至中等。在评估一名观察者的可靠性时，组内相关系数（0.95）极佳。
