Zhang Ping, Li Wei, Wang Yaochun, Hou Lihong, Xing Ying, Qin Hongyan, Wang Jishu, Liang Yingmin, Han Hua
State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, and Department of Dermatology, Xijng Hospital, Xi'an 710032, China.
Mol Immunol. 2007 Jan;44(4):332-7. doi: 10.1016/j.molimm.2006.02.030. Epub 2006 Apr 17.
Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, and CD49e were expressed on MZ B cells but not on FO B cells. In addition, using semi-quantitative PCR, we found that the mRNA of CD131 were much more abundant in MZ B cells. These results revealed new phenotypic properties of MZ and FO B cells, and would facilitate further studies in the differentiation and functions of these mature B cells.
滤泡（FO）B细胞和边缘区（MZ）B细胞属于小鼠脾脏中的成熟B细胞群体。为了鉴定这些成熟B细胞亚群的新表面标志物，我们使用流式细胞术分选的小鼠FO和MZ B细胞，通过DNA微阵列比较了FO和MZ B细胞的基因表达谱。在14000个小鼠基因中，27种膜蛋白主要在MZ B细胞中表达，而另外22种膜蛋白主要在FO B细胞中表达。通过流式细胞术分析，我们确定CD36、CD68和CD49e在MZ B细胞上表达，而在FO B细胞上不表达。此外，使用半定量PCR，我们发现CD131的mRNA在MZ B细胞中更为丰富。这些结果揭示了MZ和FO B细胞的新表型特性，并将有助于进一步研究这些成熟B细胞的分化和功能。
