Wang Zhi, Wang Hui, Rhie Julie K, Covey Joseph M, Liang Ping, Wang Shuyi, Wang Chun, Hu Yanxue, Prasad Gautam, Nan Li, Hill Donald L, Zhang Ruiwen
Department of Pharmacology and Toxicology, Division of Clinical Pharmacology, and Comprehensive Cancer Center, University of Alabama at Birmingham, 35294-0019, USA.
J Pharm Biomed Anal. 2006 Sep 18;42(2):272-6. doi: 10.1016/j.jpba.2006.02.047. Epub 2006 Apr 17.
A novel reversed-phase high-performance liquid chromatographic (HPLC) method has been established for the determination of a newly developed anti-cancer agent desoxyepothilone B (dEpo B) in nude mice plasma. The sample preparation involved deproteination of 200 microl of plasma sample first, followed by liquid-liquid extraction of the resultant supernatant with chloroform. The compound taxol was used as the internal standard. Chromatographic separations were carried out on a 250 mm x 4.6 mm Zorbax SB-phenyl column with acetonitrile-0.25% orthophosphoric acid (50/50, v/v) as mobile phase and UV detection at 250 nm. For dEpo B and taxol at the concentration level of 10 microg/ml in nude mice plasma, the absolute extraction recoveries were 85.3 and 87.2%, respectively. The linear quantification range of the method was 0.1-100 microg/ml in nude mice plasma with linear correlation coefficients greater than 0.999. The within-day and between-day relative standard deviations (R.S.D.s) for dEpo B at 0.5, 2.5 and 10 microg/ml levels in nude mice plasma fell in the range of 2.8-4.8 and 1.5-4.6%, and the within-day and between-day recoveries were in the range of 96.5-101.7 and 97.7-101.2%, respectively.
已建立一种新型反相高效液相色谱（HPLC）法，用于测定裸鼠血浆中一种新开发的抗癌药物脱氧埃坡霉素B（dEpo B）。样品制备首先是对200微升血浆样品进行去蛋白处理，然后用氯仿对所得上清液进行液-液萃取。化合物紫杉醇用作内标。色谱分离在一根250 mm×4.6 mm的Zorbax SB-苯基柱上进行，以乙腈-0.25%正磷酸（50/50，v/v）作为流动相，并在250 nm处进行紫外检测。对于裸鼠血浆中浓度为10微克/毫升的dEpo B和紫杉醇，绝对萃取回收率分别为85.3%和87.2%。该方法在裸鼠血浆中的线性定量范围为0.1 - 100微克/毫升，线性相关系数大于0.999。裸鼠血浆中dEpo B在0.5、2.5和10微克/毫升水平的日内和日间相对标准偏差（R.S.D.s）分别在2.8 - 4.8%和1.5 - 4.6%范围内，日内和日间回收率分别在96.5 - 101.7%和97.7 - 101.2%范围内。
