Tabarani Georges, Reina José J, Ebel Christine, Vivès Corinne, Lortat-Jacob Hugues, Rojo Javier, Fieschi Franck
Institut de Biologie Structurale, UMR 5075 CEA/CNRS/Université Joseph Fourier, 41, rue Jules Horowitz, 38027 Grenoble Cedex 1, France.
FEBS Lett. 2006 May 1;580(10):2402-8. doi: 10.1016/j.febslet.2006.03.061. Epub 2006 Mar 31.
DC-SIGN (dendritic cell-specific ICAM-3 grabbing non-integrin) is a C-type lectin receptor of dendritic cells and is involved in the initial steps of numerous infectious diseases. Surface plasmon resonance has been used to study the affinity of a glycodendritic polymer with 32 mannoses, to DC-SIGN. This glycodendrimer binds to DC-SIGN surfaces in the submicromolar range. This binding depends on a clustered organization of DC-SIGN mimicking its natural organization as microdomain in the dendritic cells plasma membrane. Moreover, this compound inhibits DC-SIGN binding to the HIV glycoprotein gp120 with an IC50 in the micromolar range and therefore can be considered as a potential antiviral drug.
DC-SIGN（树突状细胞特异性细胞间黏附分子-3抓取非整合素）是树突状细胞的一种C型凝集素受体，参与多种传染病的初始阶段。表面等离子体共振已被用于研究一种具有32个甘露糖的糖树枝状聚合物与DC-SIGN的亲和力。这种糖树枝状大分子在亚微摩尔范围内与DC-SIGN表面结合。这种结合依赖于DC-SIGN的簇状组织，该组织模仿其在树突状细胞质膜中作为微结构域的天然组织。此外，该化合物在微摩尔范围内以IC50抑制DC-SIGN与HIV糖蛋白gp120的结合，因此可被视为一种潜在的抗病毒药物。
