Bao Y, Lies D P, Fu H, Roberts G P
Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.
Gene. 1991 Dec 20;109(1):167-8. doi: 10.1016/0378-1119(91)90604-a.
A system is described for the single-copy, stable insertion of cloned DNA sequences into the chromosomes of Gram- bacteria. Two narrow-host-range plasmids form the basis of this system: the 'carrier' plasmid contains the mini Tn7-Km transposon, into which foreign DNA can be cloned; the 'helper' plasmid provides the Tn7 transposition functions in trans. Both plasmids are readily transferred into Gram- bacteria by conjugation. The functionality of this system has been demonstrated in Rhodospirillum rubrum.
本文描述了一种用于将克隆的DNA序列单拷贝稳定插入革兰氏阴性菌染色体的系统。该系统基于两种窄宿主范围的质粒：“载体”质粒包含mini Tn7-Km转座子，可将外源DNA克隆到其中；“辅助”质粒提供反式作用的Tn7转座功能。两种质粒都可以通过接合作用轻松转移到革兰氏阴性菌中。该系统的功能已在深红红螺菌中得到证实。
