Barry G F
Microbiology Group, Monsanto Company, St. Louis, MO 63198.
Gene. 1988 Nov 15;71(1):75-84. doi: 10.1016/0378-1119(88)90079-0.
A deletion derivative of transposon Tn7 containing the Escherichia coli lacZY genes as a selectable marker for insertion of foreign DNA into the chromosomes of soil bacteria was improved to facilitate the cloning of additional genes and their insertion by this element. This report describes a series of plasmid vectors that enable this cloning to be carried out in small, high-copy, narrow host-range plasmids. The final Tn element can then be easily moved (by transposition) without further use of restriction enzymes, to plasmids suitable for delivering it to the bacterial chromosome. The very high specificity for insertion of Tn7 into single locations in bacterial chromosomes has been exploited in the construction of a shuttle system for delivering these Tn7 elements.
转座子Tn7的一种缺失衍生物含有大肠杆菌lacZY基因，作为将外源DNA插入土壤细菌染色体的选择标记，该衍生物经过改进以促进额外基因的克隆及其通过该元件的插入。本报告描述了一系列质粒载体，可使这种克隆在小型、高拷贝、窄宿主范围的质粒中进行。然后，最终的Tn元件可以很容易地（通过转座）转移，而无需进一步使用限制酶，转移到适合将其递送至细菌染色体的质粒中。Tn7插入细菌染色体单一位置的极高特异性已被用于构建用于递送这些Tn7元件的穿梭系统。
