Zhang Yalou, Bart Jean-Mathieu, Giraudoux Patrick, Craig Philip, Vuitton Dominique, Wen Hao
Xinjiang Hydatid Clinical Research Institute, First Teaching Hospital, Xinjiang Medical University, Urumqi 830054, PR China [corrected]
Vet Parasitol. 2006 Jun 30;139(1-3):244-8. doi: 10.1016/j.vetpar.2006.03.003. Epub 2006 Apr 17.
The Xinjiang plateau of western China has been shown to have a high prevalence for human cystic echinococcosis (CE) caused by Echinococcus granulosus, and human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. The domestic dog is suspected to be the primary definitive host for the transmission of both E. granulosus and E. multilocularis to humans in this locality. Seventeen of 30 stray dogs from Hejing County of Xinjiang were found positive for E. granulosus post mortem, and one double infection was suspected. Worm samples were collected, dyed by carmine, and observed microscopically. Carmine staining examination clearly revealed the differences in number of proglottids and appearance of uterine branches and lateral genital pore for those two species of Echinococcus. Furthermore, gene target DNA fragments were amplified for formal identification of the two parasite species, based on 12s rRNA mitochondrial gene. The PCR products were purified and sequenced. Compared with NCBI GenBank, the DNA sequences demonstrated 100% identity with E. granulosus (sheep strain, G1 genotype) and E. multilocularis.
中国西部的新疆高原地区已被证实，由细粒棘球绦虫引起的人体囊型包虫病（CE）以及由多房棘球绦虫引起的人体泡型包虫病（AE）的患病率很高。家犬被怀疑是该地区细粒棘球绦虫和多房棘球绦虫向人类传播的主要终末宿主。新疆和静县30只流浪狗中有17只在死后被检测出细粒棘球绦虫呈阳性，疑似一例双重感染。采集虫体样本，用洋红染色后进行显微镜观察。洋红染色检查清楚地揭示了这两种棘球绦虫的节片数量、子宫分支外观和侧生殖孔的差异。此外，基于12s rRNA线粒体基因，扩增基因靶DNA片段以对这两种寄生虫进行正式鉴定。对PCR产物进行纯化和测序。与NCBI基因库相比，DNA序列与细粒棘球绦虫（绵羊株，G1基因型）和多房棘球绦虫的序列一致性为100%。
