Hassanain Mohey Abdelhafez, Shaapan Raafat Mohamed, Khalil Fathia Abdelrazik M
Department of Zoonotic Diseases, National Research Center, P.O. 12622, El-Tahrir Street, Dokki, Giza, Egypt.
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
J Parasit Dis. 2016 Mar;40(1):52-6. doi: 10.1007/s12639-014-0443-5. Epub 2014 Feb 22.
Cystic echinococcosis (CE) is a severe zoonotic disease which affects both human and animals. The disease has a considerable economic and social impact, because it has numerous complications leading to important disabilities and even death. CE is a widespread chronic endemic helminthic disease caused by infection with metacestodes of tapeworm Echinococcus granulosus. This study was conducted to diagnosis human CE by hydatid cyst antigens from camels and sheep. Hydatid fluid and protoscoleces crude antigens corresponding to camel and sheep were resolute by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and the protein bands of different antigens were exposed to infected patients serum CE through western blot (WB) assay. The camel hydatid fluid antigen revealed five polypeptide bands of 18-98.8 kDa by SDS-PAGE while sheep hydatid fluid antigen revealed four polypeptide bands of 20-100 kDa. Immune reactive bands were obtained through WB ranged from 25 to 125 kDa. The study showed prominent immune reactive bands of 92, 52.2 and 35.7 kDa which may helpful in diagnosis of human CE.
囊型包虫病（CE）是一种严重的人畜共患病，会影响人类和动物。该疾病具有相当大的经济和社会影响，因为它有许多并发症，会导致严重残疾甚至死亡。CE是一种广泛传播的慢性地方性蠕虫病，由感染细粒棘球绦虫的中绦期幼虫引起。本研究旨在通过骆驼和绵羊的包虫囊肿抗原诊断人类CE。在还原条件下，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）解析骆驼和绵羊对应的包虫囊液和原头节粗抗原，并通过蛋白质印迹（WB）分析将不同抗原的蛋白条带与CE感染患者血清进行接触。骆驼包虫囊液抗原经SDS-PAGE显示出五条18 - 98.8 kDa的多肽条带，而绵羊包虫囊液抗原显示出四条20 - 100 kDa的多肽条带。通过WB获得的免疫反应条带范围为25至125 kDa。该研究显示出92、52.2和35.7 kDa的显著免疫反应条带，这可能有助于人类CE的诊断。
