Chini Valentina, Rimoldi Simona, Terova Genciana, Saroglia Marco, Rossi Federica, Bernardini Giovanni, Gornati Rosalba
Dipartimento di Biotecnologie e Scienze Molecolari, Università dell'Insubria, 3 Via Dunant, I-21100 Varese, Italy.
Gene. 2006 Jul 5;376(1):102-6. doi: 10.1016/j.gene.2006.02.010. Epub 2006 Apr 17.
The scarcity of the genomic resources for some fish species, in spite of their commercial interest, could retard the positive effects that modern biotechnology can offer to aquaculture industry. Then an effort should be made to reduce, as far as it concerns genomic resources, the gap that separates farming species from "model organisms". In this paper, we present an EST project in which we performed single pass sequencing on 1229 randomly selected clones from a sea bass cDNA library. The sequences are deposited in the NCBI database with the following accession numbers: from , from , from and from . EST cataloguing and profiling of seabass will set the basis for functional genomic research in this species, but will also serve for comparative and environmental genomics, for the identification of polymorphic markers useful, for example, to survey the disease resistance of fish, for the discovery of new molecular markers of exposure and for the production of micro- and macro-arrays.
尽管某些鱼类具有商业价值，但它们的基因组资源却很稀缺，这可能会阻碍现代生物技术为水产养殖业带来的积极影响。因此，应努力缩小养殖鱼类与“模式生物”在基因组资源方面的差距。在本文中，我们展示了一个EST项目，在该项目中，我们对从海鲈cDNA文库中随机选择的1229个克隆进行了单向测序。这些序列已存入NCBI数据库，登录号如下：从……，从……，从……和从……。海鲈的EST编目和分析将为该物种的功能基因组研究奠定基础，也将用于比较基因组学和环境基因组学，用于鉴定例如用于调查鱼类抗病性的多态性标记、发现新的暴露分子标记以及生产微阵列和宏阵列。
