Liu Hong Tao, Gao Fei, Cui Shu Juan, Han Jin Long, Sun Da Ye, Zhou Ren Gang
Institute of Genetics and Physiology, Hebei Academy of Agricultural Sciences, Shijiazhuang 050051, China.
Cell Res. 2006 Apr;16(4):394-400. doi: 10.1038/sj.cr.7310051.
The role of inositol 1,4,5-trisphosphate (IP(3)) in transducing heat-shock (HS) signals was examined in Arabidopsis. The whole-plant IP(3) level increased within 1 min of HS at 37 degrees C. After 3 min of HS, the IP(3) level reached a maximum 2.5 fold increase. Using the transgenic Arabidopsis plants that have AtHsp18.2 promoter-beta-glucuronidase (GUS) fusion gene, it was found that the level of GUS activity was up-regulated by the addition of caged IP(3) at both non-HS and HS temperatures and was down-regulated by the phospholipase C (PLC) inhibitors {1-[6-((17beta-3-Methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]-2,5-pyrrolidinedione}(U-73122). The intracellular-free calcium ion concentration (Ca(2+)) increased during HS at 37 degrees C in suspension-cultured Arabidopsis cells expressing apoaequorin. Treatment with U-73122 prevented the increase of Ca(2+) to some extent. Above results provided primary evidence for the possible involvement of IP(3) in HS signal transduction in higher plants.
在拟南芥中研究了肌醇1,4,5 - 三磷酸（IP(3)）在转导热激（HS）信号中的作用。在37℃热激1分钟内，整株植物的IP(3)水平升高。热激3分钟后，IP(3)水平达到最大值，增加了2.5倍。使用具有AtHsp18.2启动子 - β - 葡萄糖醛酸酶（GUS）融合基因的转基因拟南芥植株，发现在非热激和热激温度下，加入笼化IP(3)均可上调GUS活性水平，而磷脂酶C（PLC）抑制剂{1 - [6 - ((17β - 3 - 甲氧基雌甾 - 1,3,5(10) - 三烯 - 17 - 基)氨基)己基] - 2,5 - 吡咯烷二酮}(U - 73122)可下调其活性水平。在表达水母发光蛋白的悬浮培养拟南芥细胞中，37℃热激期间细胞内游离钙离子浓度（Ca(2+)）升高。用U - 73122处理在一定程度上可阻止Ca(2+)的升高。上述结果为IP(3)可能参与高等植物热激信号转导提供了初步证据。
