Chen Jing, Pang Xue-wen, Liu Fang-fang, Dong Xue-yuan, Wang Hong-cheng, Wang Shan, Zhang Yu, Chen Wei-feng
Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Apr 18;38(2):124-7.
To investigate PLAC1/CP1 as a potential candidate gene in gastric cancer therapy.
Reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the expression of PLAC1/CP1 mRNA in gastric cancer tissues and paired adjacent non-cancerous tissues resected from 28 patients with gastric cancer. Specific antibodies against PLAC1/CP1 were also detected by ELISA method in gastric cancer patients.
Fourteen (50%,14/28) out of the 28 gastric cancer samples were PLAC1/CP1 mRNA positive. Of the 28 serum samples tested,eight (29%,8/28) displayed positive seroreactivity against PLAC1/CP1 antigen, accounting for 57% (8/14) of PLAC1/CP1 mRNA positive samples.
PLAC1/CP1 mRNA was expressed in high frequency and induced spontaneous antibody responses in gastric cancer patients. PLAC1/CP1 may be a valuable candidate antigen in gastric cancer immunotherapy.
研究PLAC1/CP1作为胃癌治疗潜在候选基因的情况。
采用逆转录-聚合酶链反应（RT-PCR）法检测28例胃癌患者手术切除的癌组织及配对的癌旁正常组织中PLAC1/CP1 mRNA的表达。采用酶联免疫吸附测定（ELISA）法检测胃癌患者血清中针对PLAC1/CP1的特异性抗体。
28例胃癌组织样本中，14例（50%，14/28）PLAC1/CP1 mRNA呈阳性。28例血清样本中，8例（29%，8/28）对PLAC1/CP1抗原呈阳性血清反应，占PLAC1/CP1 mRNA阳性样本的57%（8/14）。
PLAC1/CP1 mRNA在胃癌患者中高频率表达并诱导自发抗体反应。PLAC1/CP1可能是胃癌免疫治疗中有价值的候选抗原。
