Mashayekhi F, Salehi Z
Department of Biology, Faculty of Sciences, The University of Guilan, Rasht, Iran.
Eur J Neurol. 2006 Mar;13(3):266-72. doi: 10.1111/j.1468-1331.2006.01208.x.
Cerebrospinal fluid (CSF) is mainly produced by the choroid plexuses within the ventricles of the brain. The CSF circulates in a regular manner after the ventricular system and the choroids plexuses have developed, and the foramina in the fourth ventricle have opened to enable it to carry chemical information. CSF flows through the ventricular system passing over all regions of germinal activity. In this study, chick embryos were used to show the importance of CSF on neural cell proliferation in the developing cerebral cortex. The chick embryos were cannulated in situ with a fine capillary tube to drain CSF out of the ventricular system. At the same time, BrdU was administered to the embryos. After surgery the embryos were incubated for another 3 days. Quantitative measurements showed that the thicknesses of the germinal epithelium and cerebral cortex in CSF-drained embryos were less than those in the control group at the same age. The number of cells produced in the germinal epithelium of CSF-drained embryos was decreased when compared with the normal group. This study provides confirmatory evidence that CSF is important for neural cell proliferation and therefore normal development of the cerebral cortex. It is proposed that CSF is vital in controlling development of the cerebral cortex.
脑脊液（CSF）主要由脑室内的脉络丛产生。在脑室系统和脉络丛发育完成且第四脑室的孔道开放后，脑脊液以规则的方式循环，使其能够携带化学信息。脑脊液流经脑室系统，覆盖所有生发活动区域。在本研究中，使用鸡胚来展示脑脊液对发育中的大脑皮质神经细胞增殖的重要性。用细毛细管对鸡胚进行原位插管，以将脑脊液从脑室系统中引流出来。同时，给胚胎注射溴脱氧尿苷（BrdU）。手术后，将胚胎再孵化3天。定量测量表明，引流脑脊液的胚胎中生发上皮和大脑皮质的厚度小于同年龄对照组。与正常组相比，引流脑脊液的胚胎生发上皮中产生的细胞数量减少。本研究提供了确凿证据，证明脑脊液对神经细胞增殖以及大脑皮质的正常发育很重要。有人提出，脑脊液在控制大脑皮质发育方面至关重要。
