Erwin Christopher R, Jarboe Marcus D, Sartor Maureen A, Medvedovic Mario, Stringer Keith F, Warner Brad W, Bates Michael D
Division of Pediatric and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.
Gastroenterology. 2006 Apr;130(4):1324-32. doi: 10.1053/j.gastro.2006.02.019.
BACKGROUND & AIMS: Following massive small bowel resection (SBR), the remnant intestine undergoes an adaptive process characterized by increases in a number of physiologic and morphologic parameters. These changes are the result of a stimulus that increases crypt cell mitosis and augments cellular progression along the villus axis. To better define this process, we identified patterns of gene expression specifically within adapting intestinal crypt cells following SBR.
Laser capture microdissection was used to isolate mouse intestinal crypt cells following SBR or sham operation. Multiple biological and technical complementary DNA microarray replicates allowed rigorous statistical analyses for identification of important expression profiles. Major groups of genes were classified as to site of action, functional pathway, and possible regulatory groups.
A total of 300 genes differentially expressed at significant levels within adapting crypt enterocytes were analyzed. Comparison of this list of differentially expressed adapting crypt cell genes with a generalized mouse gene expression database (from 82 developing and adult mouse tissues) showed the greatest overlap with developing and immature intestinal tissues. We identified prominent groups of genes involved with cell growth, signal transduction, and nucleic acid binding. Genes not previously shown to be involved with adaptation or development and maturation were identified.
Identification of similar genes coordinately regulated during both adaptation and development, processes that share key morphologic features, provides a basis for new mechanistic insights into these shared characteristics.
在进行大规模小肠切除（SBR）后，残余肠道会经历一个适应性过程，其特征是一些生理和形态学参数增加。这些变化是一种刺激的结果，该刺激会增加隐窝细胞的有丝分裂，并促进细胞沿绒毛轴的进展。为了更好地定义这一过程，我们确定了SBR后适应性肠隐窝细胞内特定的基因表达模式。
使用激光捕获显微切割技术分离SBR或假手术后的小鼠肠隐窝细胞。多个生物学和技术互补DNA微阵列复制品允许进行严格的统计分析，以识别重要的表达谱。主要基因组根据作用位点、功能途径和可能的调控组进行分类。
共分析了300个在适应性隐窝肠上皮细胞中显著差异表达的基因。将这份差异表达的适应性隐窝细胞基因列表与一个广义的小鼠基因表达数据库（来自82个发育中和成年小鼠组织）进行比较，发现与发育中和未成熟的肠道组织重叠最大。我们确定了涉及细胞生长、信号转导和核酸结合的突出基因组。还发现了以前未显示与适应、发育和成熟有关的基因。
在适应和发育过程中协调调控的相似基因的鉴定，这两个过程具有关键的形态学特征，为对这些共同特征的新机制见解提供了基础。
