Yang Yi, Hou Ying, Wang Chang-Lin, Ji Shi-Jun
Department of Pediatric Surgery, Second Affiliated Hospital of China Medical University, Shenyang City, China.
Urology. 2006 Apr;67(4):817-21; discussion 821-2. doi: 10.1016/j.urology.2005.10.062.
To study the potential role of pelviureteral junction obstruction (PUJO) in causing progressive renal damage in children through the renal expression of epidermal growth factor (EGF) and transforming growth factor-beta1 (TGF-beta1).
The expression of EGF and TGF-beta1 was evaluated in the renal tissues of 25 children with congenital hydronephrosis by immunohistochemistry, in situ hybridization, and reverse transcriptase polymerase chain reaction techniques.
Children with PUJO had a significant increase in TGF-beta1 and a marked reduction in EGF expression compared with controls. The TGF-beta1/glyceraldehyde phosphate dehydrogenase ratio in the hydronephrotic kidney and normal kidney was 0.53 +/- 0.13 and 0.24 +/- 0.10 respectively, and the difference was significant (P = 0.000). The EGF/glyceraldehyde phosphate dehydrogenase ratio in the hydronephrotic kidney and normal kidney was 0.15 +/- 0.06 and 0.55 +/- 0.13, respectively, and the difference was also significant (P = 0.0001). Positive correlations were found between the TGF-beta1 gene and the drainage clearance half-time (r = 0.47; P = 0.018), TGF-beta1 protein and drainage clearance half-time (r = 0.44; P = 0.028), TGF-beta1 gene and histologic grade (r = 0.53; P = 0.006), and TGF-beta1 protein and histologic grade (r = 0.76; P = 0.000). Negative correlations were found between the EGF gene and drainage clearance half-time (r = -0.59; P = 0.002), EGF protein and drainage clearance half-time (r = -0.61; P = 0.001), EGF gene and histologic grade (r = -0.58; P = 0.003), and EGF protein and histologic grade (r = -0.47; P = 0.019).
TGF-beta1 expression was increased and EGF expression was decreased in the renal tissue after clinical PUJO. The alterations of TGF-beta1 and EGF may play a potential role in the pathogenesis of renal damage in PUJO.
通过表皮生长因子（EGF）和转化生长因子-β1（TGF-β1）在肾脏中的表达，研究肾盂输尿管连接部梗阻（PUJO）在儿童进行性肾损害中的潜在作用。
采用免疫组织化学、原位杂交和逆转录聚合酶链反应技术，评估25例先天性肾积水患儿肾组织中EGF和TGF-β1的表达。
与对照组相比，PUJO患儿TGF-β1表达显著增加，EGF表达明显降低。肾积水肾脏和正常肾脏中TGF-β1/甘油醛-3-磷酸脱氢酶比值分别为0.53±0.13和0.24±0.10，差异有统计学意义（P = 0.000）。肾积水肾脏和正常肾脏中EGF/甘油醛-3-磷酸脱氢酶比值分别为0.15±0.06和0.55±0.13，差异也有统计学意义（P = 0.0001）。TGF-β1基因与引流清除半衰期呈正相关（r = 0.47；P = 0.018），TGF-β1蛋白与引流清除半衰期呈正相关（r = 0.44；P = 0.028），TGF-β1基因与组织学分级呈正相关（r = 0.53；P = 0.006），TGF-β1蛋白与组织学分级呈正相关（r = 0.76；P = 0.000）。EGF基因与引流清除半衰期呈负相关（r = -0.59；P = 0.002），EGF蛋白与引流清除半衰期呈负相关（r = -0.61；P = 0.001），EGF基因与组织学分级呈负相关（r = -0.58；P = 0.003），EGF蛋白与组织学分级呈负相关（r = -0.47；P = 0.019）。
临床PUJO后肾组织中TGF-β1表达增加，EGF表达降低。TGF-β1和EGF的改变可能在PUJO肾损害的发病机制中起潜在作用。
