通过广谱聚合酶链反应检测克罗恩病中的分枝杆菌
Detection of mycobacteria in Crohn's disease by a broad spectrum polymerase chain reaction.
作者信息
Tzen Chi-Yuan, Wu Tsu-Yen, Tzen Chin-Yuan
机构信息
Department of Pathology, Mackay Memorial Hospital, National Taipei College of Nursing, and Mackay Medicine, Taipei, Taiwan.
出版信息
J Formos Med Assoc. 2006 Apr;105(4):290-8. doi: 10.1016/S0929-6646(09)60120-0.
BACKGROUND
The role of mycobacterial infection, particularly related to Mycobacterium avium subsp paratuberculosis (Map), in Crohn's disease has long been debated. We developed primer pairs capable of detecting a broad spectrum of mycobacterium and employed them to investigate surgical specimens from patients with Crohn's disease.
METHODS
Pan mycobacterium primers of the 65-kDa heat shock protein gene (Hsp65) were used in a polymerase chain reaction (PCR) to examine 12 surgically-resected, formalin-fixed, paraffin-embedded specimens from 11 patients with Crohn's disease. The DNA sequences of amplicons were aligned with those in GenBank.
RESULTS
Mycobacterial DNA was found in specimens from three of 11 patients. M. mucogenicum was identified in a specimen from one patient and M. tuberculosis in two, but Map was not identified in any.
CONCLUSION
Hsp65-based PCR can be employed to search for occult mycobacterial infection of the gastrointestinal tract in patients with a diagnosis or suspicion of Crohn's disease. This approach may have a therapeutic implication.
背景
分枝杆菌感染，尤其是与副结核分枝杆菌（Map）相关的感染在克罗恩病中的作用长期以来一直存在争议。我们开发了能够检测多种分枝杆菌的引物对，并用于研究克罗恩病患者的手术标本。
方法
使用65 kDa热休克蛋白基因（Hsp65）的泛分枝杆菌引物进行聚合酶链反应（PCR），以检测11例克罗恩病患者的12份手术切除、福尔马林固定、石蜡包埋的标本。扩增子的DNA序列与GenBank中的序列进行比对。
结果
在11例患者中的3例标本中发现了分枝杆菌DNA。在1例患者的标本中鉴定出黏液产色分枝杆菌，2例中鉴定出结核分枝杆菌，但未在任何标本中鉴定出Map。
结论
基于Hsp65的PCR可用于在诊断或怀疑患有克罗恩病的患者中寻找胃肠道隐匿性分枝杆菌感染。这种方法可能具有治疗意义。
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