Slagter Margrita H, Gooren Louis J G, Scorilas Andreas, Petraki Constantina D, Diamandis Eleftherios P
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, M5G 1X5, Canada.
J Histochem Cytochem. 2006 Aug;54(8):905-10. doi: 10.1369/jhc.6A6928.2006. Epub 2006 Apr 17.
Our aim was to examine the effects of androgen administration on breast tissue histology of female-to-male transsexuals and to study the immunohistochemical expression of three human tissue kallikreins, hK3 (PSA), hK6, and hK10. We studied 23 female-to-male transsexuals who were treated with injectable testosterone for 18-24 months. We also used 10 control female breast tissues. All tissues were fixed in buffered formalin, embedded in paraffin, and examined by hematoxylin-eosin staining and immunohistochemical staining for PSA, hK6, and hK10. Females treated with androgens exhibited similar involutionary changes as those seen in breast of menopausal women, such as marked reduction of glandular tissue, involution of the lobuloalveolar structures, and prominence of fibrous connective tissue, but presence of only small amounts of fat tissue. Fibrocystic lesions were generally not observed. In immunohistochemistry, in control breast tissues, we found moderate to strong cytoplasmic immunoexpression of hK6 and hK10 in the epithelial ductal and lobuloalveolar structures, but myoepithelial cells were negative. Luminal secretions were also positive. In menopausal breast, the immunoexpression of hK6 and hK10 was weaker and focal. No control case showed immunoexpression for PSA. In female-to-male transsexuals, one case showed focal PSA cytoplasmic immunoexpression in the epithelium of moderately involuting lobules. Long-term administration of androgens in female-to-male transsexuals causes marked reduction of glandular tissue and prominence of fibrous connective tissue. These changes are similar to those observed at the end-stage of menopausal mammary involution.
我们的目的是研究雄激素给药对女变男跨性别者乳腺组织组织学的影响，并研究三种人组织激肽释放酶hK3（前列腺特异性抗原，PSA）、hK6和hK10的免疫组化表达。我们研究了23名接受注射用睾酮治疗18 - 24个月的女变男跨性别者。我们还使用了10例对照女性乳腺组织。所有组织均用缓冲福尔马林固定，石蜡包埋，并通过苏木精 - 伊红染色以及PSA、hK6和hK10的免疫组化染色进行检查。接受雄激素治疗的女性表现出与绝经后女性乳腺相似的 involutionary 变化，如腺组织明显减少、小叶腺泡结构退化以及纤维结缔组织突出，但仅存在少量脂肪组织。一般未观察到纤维囊性病变。在免疫组化中，在对照乳腺组织中，我们发现hK6和hK10在上皮导管和小叶腺泡结构中呈中度至强细胞质免疫表达，但肌上皮细胞呈阴性。管腔分泌物也呈阳性。在绝经后乳腺中，hK6和hK10的免疫表达较弱且呈局灶性。没有对照病例显示PSA免疫表达。在女变男跨性别者中，1例在中度退化小叶的上皮中显示局灶性PSA细胞质免疫表达。长期给予女变男跨性别者雄激素会导致腺组织明显减少和纤维结缔组织突出。这些变化与绝经后乳腺 involution 末期观察到的变化相似。
