Burgess H E, Shousha S
Department of Histopathology, Charing Cross and Westminister Medical School, London, U.K.
J Pathol. 1993 May;170(1):37-43. doi: 10.1002/path.1711700107.
This study was aimed at assessing the effects of therapeutic long-term administration of androgens on normal human female breast. Sections from mastectomy specimens of 29 female-to-male transsexuals who had prolonged androgen administration prior to surgery were examined using routine light microscopy and immunohistochemical techniques. For comparison, sections from ten 'normal' female breast reduction mammoplasty specimens and ten cases of gynaecomastia were similarly examined. Haematoxylin and eosin-stained sections were assessed for the prevalence of elements of the normal breast and benign breast lesions. Immunoperoxidase techniques were performed to study the distribution of a variety of breast-associated antigens and receptors. The results were assessed semi-quantitatively. The prevalence of normal acini and ducts, fibrosis, cysts, and apocrine metaplasia in transsexual specimens was not statistically different from that seen in normal controls. However, transsexual specimens had a significantly higher prevalence of microcalcification than normals. The majority of transsexual specimens were positive for gross cystic disease fluid protein-15, lactoferrin, and progesterone and oestrogen receptors, and negative for B72.3 and pS2. These findings were not significantly different from those in normal controls. All ten gynaecomastia specimens were positive for oestrogen and progesterone receptors. The prevalence of oestrogen receptors was significantly higher than that seen in transsexuals and normal controls, but the prevalence of progesterone receptors was only significantly higher than that seen in transsexuals. It is concluded that long-term androgen administration does not appear to have any significant lasting effect on the normal human female breast, as demonstrated by a wide range of histological and immunohistological criteria.
本研究旨在评估长期给予雄激素治疗对正常女性乳房的影响。对29例在手术前长期接受雄激素治疗的女性变男性易性癖者的乳房切除标本切片，采用常规光学显微镜和免疫组织化学技术进行检查。作为对照，对10例“正常”女性乳房缩小整形标本和10例男性乳房肥大病例的切片进行同样检查。苏木精和伊红染色切片用于评估正常乳腺成分和良性乳腺病变的发生率。采用免疫过氧化物酶技术研究多种乳腺相关抗原和受体的分布。结果进行半定量评估。易性癖者标本中正常腺泡和导管、纤维化、囊肿及大汗腺化生的发生率与正常对照相比无统计学差异。然而，易性癖者标本中微钙化的发生率显著高于正常人。大多数易性癖者标本中，大体囊肿病液体蛋白-15、乳铁蛋白、孕激素和雌激素受体呈阳性，而B72.3和pS2呈阴性。这些结果与正常对照无显著差异。所有10例男性乳房肥大标本中雌激素和孕激素受体均呈阳性。雌激素受体的发生率显著高于易性癖者和正常对照，但孕激素受体的发生率仅显著高于易性癖者。结论是，从广泛的组织学和免疫组织学标准来看，长期给予雄激素似乎对正常女性乳房没有任何显著的持久影响。
