Arango Eliana, Londoño Berlin, Segura Cesar, Solarte Yezid, Herrera Socrates, Saez Jairo, Carmona-Fonseca Jaime, Blair Silvia
Grupo Malaria, Universidad de Antioquia, Medellín, Colombia.
Phytother Res. 2006 Jun;20(6):444-7. doi: 10.1002/ptr.1874.
The sporontocidal activity of three steroids (SN-1, SN-2 and SN-4) from Solanum nudum Dunal (Solanaceae) was determined against naturally circulating isolates of Plasmodium vivax in Anopheles albimanus. Laboratory-reared Anopheles albimanus mosquitoes were infected with P. vivax from gametocytemic blood of volunteers resident in Buenaventura, Valle del Cauca (Colombian Pacific Coast) by using an artificial membrane feeder. Prior to mosquito feeding, gametocytemic blood was centrifuged, plasma was separated, packed blood red cells were washed with RPMI 1640 and then resuspended in non-immune AB serum, then the steroids were added at different doses. On day 7 after infection, the presence and number of oocysts in mosquitoes was determined. The steroid SN-2 reduced the infection of mosquitoes by 90% and the mean number of oocysts by 60%. These data confirmed that the experimental steroid is capable of interrupting the sporogonic development of P. vivax in Anopheles albimanus. This experimental steroid has potential for transmission blocking in vivax malaria.
测定了来自茄科野茄（Solanum nudum Dunal）的三种甾体（SN-1、SN-2和SN-4）对白纹伊蚊体内自然传播的间日疟原虫分离株的杀孢子体活性。通过使用人工膜饲器，用来自哥伦比亚太平洋沿岸考卡山谷布埃纳文图拉常住志愿者的配子体血症血液感染实验室饲养的白纹伊蚊。在蚊虫取食前，将配子体血症血液离心，分离血浆，用RPMI 1640洗涤压积红细胞，然后重悬于非免疫AB血清中，随后加入不同剂量的甾体。感染后第7天，测定蚊虫体内卵囊的存在情况和数量。甾体SN-2使蚊虫感染率降低了90%，卵囊平均数降低了60%。这些数据证实，该实验性甾体能够阻断间日疟原虫在白纹伊蚊体内的孢子生殖发育。这种实验性甾体在间日疟的传播阻断方面具有潜力。
