人嗜神经JC病毒在结肠癌细胞中的调控
Regulation of human neurotropic JCV in colon cancer cells.
作者信息
Enam Sahnila, Gan Dai-Di, White Martyn K, Del Valle Luis, Khalili Kamel
机构信息
Center for Neurovirology, Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA 19122, USA.
出版信息
Anticancer Res. 2006 Mar-Apr;26(2A):833-41.
BACKGROUND
Recently, the genome of the human polyomavirus, JC (JCV), and expression of its early and late regulatory proteins (T-antigen and agnoprotein) have been demonstrated in neoplastic cells of colonic cancer cases.
MATERIALS AND METHODS
Regulation of JCV was investigated in a human colon cancer cell line (SW480) and compared to a human glioblastoma cell line (U87-MG) that is permissive for JCV replication.
RESULTS
SW480 cells supported basal transcription of both early and late JCV promoters. The expression of TCF-4, a component of Wnt signaling, modulated JCV transcription in a cell type-specific manner. Both TCF-4 and T-antigen bound to the JCV promoter region and bound to each other. In addition, the expression of TCF-4 caused a decrease in the ability of the T-antigen to stimulate viral DNA replication in U87-MG cells.
CONCLUSION
Wnt pathway signaling proteins and T-antigen interact to regulate JCV in colonic epithelial cells.
背景
最近，已在结肠癌病例的肿瘤细胞中证实了人多瘤病毒JC（JCV）的基因组及其早期和晚期调节蛋白（T抗原和agnoprotein）的表达。
材料与方法
在人结肠癌细胞系（SW480）中研究JCV的调节，并与允许JCV复制的人胶质母细胞瘤细胞系（U87-MG）进行比较。
结果
SW480细胞支持JCV早期和晚期启动子的基础转录。Wnt信号通路的一个组成部分TCF-4的表达以细胞类型特异性方式调节JCV转录。TCF-4和T抗原均与JCV启动子区域结合并相互结合。此外，TCF-4的表达导致T抗原刺激U87-MG细胞中病毒DNA复制的能力下降。
结论
Wnt信号通路信号蛋白与T抗原相互作用以调节结肠上皮细胞中的JCV。
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