Chang Chih-Hsien, Wang Hsin-Ell, Wu Shi-Yuan, Fan Kuo-Hsien, Tsai Tung-Hu, Lee Te-Wei, Chang Shiang-Rong, Liu Ren-Shyan, Chen Chieh-Fu, Chen Chin-Hsiung, Fu Ying-Kai
Institute of Pharmacology, National Yang-Ming University, Taipei, ROC.
Anticancer Res. 2006 Mar-Apr;26(2A):917-25.
Clinical FDG/PET (2-deoxy-2-18F-fluoro-D-glucose/positron emission tomography) studies encounter difficulties in detecting early stage lung cancers. The aim of this study was to evaluate the ability of O-2-18F-fluoroethyl-L-tyrosine (FET) and FDG to differentiate between inflammation and lung carcinoma in mice.
Sixty-four C57BL/6 mice were inoculated with 2x10(6) LLC1 lung carcinoma cells in the right hind flank on day 0 and were then injected with 0.1 mL turpentine in the left thigh muscle on day 3. The progress of inflammation and tumor in mice was longitudinally monitored by FDG/microPET. The biodistribution study, pharmacokinetic evaluation and whole-body autoradiography of FET and FDG were performed on day 8 after tumor inoculation.
The FDG uptakes in tumor and inflammatory lesions were 4.42-fold and 3.53-fold (n = 4) higher, respectively, than that in muscle at 90 min post-injection and the tumor-to-inflammation ratio was 1.25. For FET/microPET, the tumor uptake was 2.07-fold and 2.07-fold (n = 4) higher than those in muscle and inflammatory lesions at 90 min post-injection, respectively. The distribution half-life (t1/2,alpha) and the elimination half-life (t1/2,beta) of FET were 39 min and 205 min, respectively, in mice.
FDG delineated both tumor and inflammation, while FET accumulated in tumor to a significantly higher extent. Our results demonstrated the potential of FET to distinguish epidermoid lung carcinoma from inflammatory lesions in mice.
临床氟代脱氧葡萄糖/正电子发射断层扫描（FDG/PET）研究在检测早期肺癌时遇到困难。本研究的目的是评估O-2-18F-氟乙基-L-酪氨酸（FET）和FDG区分小鼠炎症和肺癌的能力。
64只C57BL/6小鼠于第0天在右后腹接种2×10⁶个LLC1肺癌细胞，然后于第3天在左大腿肌肉注射0.1 mL松节油。通过FDG/微型正电子发射断层扫描纵向监测小鼠炎症和肿瘤的进展。在接种肿瘤后第8天进行FET和FDG的生物分布研究、药代动力学评估和全身放射自显影。
注射后90分钟时，肿瘤和炎症病变中的FDG摄取量分别比肌肉中的高4.42倍和3.53倍（n = 4），肿瘤与炎症的比值为1.25。对于FET/微型正电子发射断层扫描，注射后90分钟时肿瘤摄取量分别比肌肉和炎症病变中的高2.07倍（n = 4）。在小鼠中，FET的分布半衰期（t1/2，α）和消除半衰期（t1/2，β）分别为39分钟和205分钟。
FDG可同时勾勒出肿瘤和炎症，而FET在肿瘤中的蓄积程度明显更高。我们的结果证明了FET在区分小鼠表皮样肺癌和炎症病变方面的潜力。
