白藜芦醇抑制胶质瘤血管生成：彩色多普勒超声评估
Resveratrol suppresses angiogenesis in gliomas: evaluation by color Doppler ultrasound.
作者信息
Chen Jin-Cherng, Chen Yun, Lin Jyh-Herng, Wu Jiann-Ming, Tseng Sheng-Hong
机构信息
Department of Surgery, Buddhist Tzu Chi Dalin General Hospital, 2, Ming Shen Road, Dalin, Chia-Yi, Taiwan, ROC.
出版信息
Anticancer Res. 2006 Mar-Apr;26(2A):1237-45.
BACKGROUND
The effects of resveratrol (a natural polyphenol) on angiogenesis in rat gliomas were investigated by color Doppler ultrasound (CDUS). The correlation among the tumor growth rate, macroscopic angiogenesis measured by CDUS and microscopic angiogenesis was assessed by immunohistochemical staining.
MATERIALS AND METHODS
Fischer rats were subcutaneously inoculated with rat RT-2 glioma cells and treated with resveratrol for 4 weeks. The tumor size was measured and the animal survival followed. CDUS examination was used to measure tumor blood flow shown as the color Doppler vascularity index (CDVI). Immunohistochemical staining of CD31 was carried out to assess the microvessel density (MVD) of the tumors. The CDVI, MVD and tumor size were correlated.
RESULTS
Rats treated with resveratrol (40 mg/kg/day) had slower tumor growth rates than those of the control groups (p < 0.05). The CDVI, MVD and tumor size were significantly correlated (linear regression, p < 0.0001).
CONCLUSION
Resveratrol-suppressed glioma growth was significantly correlated with the inhibition of macroscopic and microscopic angiogenesis.
背景
通过彩色多普勒超声（CDUS）研究白藜芦醇（一种天然多酚）对大鼠胶质瘤血管生成的影响。通过免疫组织化学染色评估肿瘤生长速率、CDUS测量的宏观血管生成与微观血管生成之间的相关性。
材料与方法
将大鼠RT-2胶质瘤细胞皮下接种于Fischer大鼠，并给予白藜芦醇治疗4周。测量肿瘤大小并跟踪动物存活情况。使用CDUS检查测量肿瘤血流，以彩色多普勒血管指数（CDVI）表示。进行CD31免疫组织化学染色以评估肿瘤的微血管密度（MVD）。对CDVI、MVD和肿瘤大小进行相关性分析。
结果
用白藜芦醇（40毫克/千克/天）治疗的大鼠肿瘤生长速率比对照组慢（p<0.05）。CDVI、MVD和肿瘤大小显著相关（线性回归，p<0.0001）。
结论
白藜芦醇抑制胶质瘤生长与抑制宏观和微观血管生成显著相关。
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