Chen Sijia, Tao Jialong, Zhong Fengyun, Jiao Yang, Xu Jiaying, Shen Qiang, Wang Haichao, Fan Saijun, Zhang Yusong
Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China.
School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu, P.R. China.
PLoS One. 2017 May 3;12(5):e0176501. doi: 10.1371/journal.pone.0176501. eCollection 2017.
Polydatin (PD), a component isolated from Polygonum cuspidatum, has a number of biological functions. However, the antitumor activity of PD has been poorly investigated. In this study, the effect of PD on cell proliferation was evaluated by thiazolyl blue tetrazolium bromide assay. Cell cycle distribution and apoptosis were investigated by flow cytometry. The phosphorylation levels of panel of phosphor-kinases were detected by human phospho-kinase arrays. The expression of several proteins associated with cell cycle and apoptosis were analyzed by Western blot analysis. Results showed that PD effectively inhibited the growth of MDA-MB-231 and MCF-7 breast cancer cell lines. Cell cycle analysis demonstrated that PD induced S-phase cell cycle arrest. Human phosphor-kinase arrays showed that the phosphorylation level of cAMP response element-bingding proteins(Creb) was down-regulated, and the results were further confirmed by Western blot analysis. Western blot analysis showed that the expression of protein of cyclin D1 decreased in a time- and dose- dependent manner. Results suggest that PD is a potential therapeutic natural compound.
虎杖苷（PD）是从虎杖中分离出的一种成分，具有多种生物学功能。然而，PD的抗肿瘤活性尚未得到充分研究。在本研究中，通过噻唑蓝溴化四氮唑法评估了PD对细胞增殖的影响。采用流式细胞术研究细胞周期分布和凋亡情况。通过人磷酸激酶阵列检测一组磷酸激酶的磷酸化水平。通过蛋白质印迹分析来分析几种与细胞周期和凋亡相关的蛋白质的表达。结果表明，PD能有效抑制MDA-MB-231和MCF-7乳腺癌细胞系的生长。细胞周期分析表明，PD诱导S期细胞周期阻滞。人磷酸激酶阵列显示，环磷酸腺苷反应元件结合蛋白（Creb）的磷酸化水平下调，蛋白质印迹分析进一步证实了该结果。蛋白质印迹分析表明，细胞周期蛋白D1的蛋白表达呈时间和剂量依赖性降低。结果表明，PD是一种具有潜在治疗作用的天然化合物。
