Kim M K, Singleton I, Yin C-R, Quan Z-X, Lee M, Lee S-T
Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea.
Lett Appl Microbiol. 2006 May;42(5):495-500. doi: 10.1111/j.1472-765X.2006.01910.x.
To study the effect of co-contaminants (phenol) on the biodegradation of pyridine by freely suspended and calcium alginate immobilized bacteria.
Varying concentrations of phenol were added to free and calcium alginate immobilized Pseudomonas putida MK1 (KCTC 12283) to examine the effect of this pollutant on pyridine degradation. When the concentration of phenol reached 0.38 g l(-1), pyridine degradation by freely suspended bacteria was inhibited. The increased inhibition with the higher phenol levels was apparent in increased lag times. Pyridine degradation was essentially completely inhibited at 0.5 g l(-1) phenol. However, immobilized cells showed tolerance against 0.5 g l(-1) phenol and pyridine degradation by immobilized cell could be achieved.
This works shows that calcium alginate immobilization of microbial cells can effectively increase the tolerance of P. putida MK1 to phenol and results in increased degradation of pyridine.
Treatment of wastewater stream can be negatively affected by the presence of co-pollutants. This work demonstrates the potential of calcium alginate immobilization of microbes to protect cells against compound toxicity resulting in an increase in pollutant degradation.
研究共污染物（苯酚）对游离悬浮和海藻酸钙固定化细菌降解吡啶的影响。
向游离的和海藻酸钙固定化的恶臭假单胞菌MK1（KCTC 12283）中添加不同浓度的苯酚，以考察该污染物对吡啶降解的影响。当苯酚浓度达到0.38 g l⁻¹时，游离悬浮细菌对吡啶的降解受到抑制。随着苯酚浓度升高，抑制作用增强，表现为延滞期延长。在苯酚浓度为0.5 g l⁻¹时，吡啶降解基本完全受到抑制。然而，固定化细胞对0.5 g l⁻¹苯酚表现出耐受性，能够实现固定化细胞对吡啶的降解。
本研究表明，海藻酸钙固定化微生物细胞可有效提高恶臭假单胞菌MK1对苯酚的耐受性，并增加吡啶的降解。
共污染物的存在可能对废水处理产生负面影响。本研究证明了海藻酸钙固定化微生物保护细胞免受复合毒性影响从而提高污染物降解的潜力。
