Juang Ruey-Shin, Wu Cheng-Ying
Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li 320, Taiwan.
Chemosphere. 2007 Jan;66(1):191-8. doi: 10.1016/j.chemosphere.2006.04.070. Epub 2006 Jun 12.
A microporous polypropylene (PP) hollow fiber membrane contactor was used as a bioreactor to degrade phenol in aqueous solutions by Pseudomonas putida BCRC 14365 at 30 degrees C. The fibers were pre-wetted by ethanol to make them more hydrophilic. The initial cell density was fixed at 0.025 gl(-1). The effects of added NaCl concentration (0-1.78 M) and pH (3-8) in substrate solution on the biodegradation were studied. The experimental results by suspended cells were discussed. It was shown that the cells in microporous hollow fibers were unable to tolerate substrate solution pH to a larger range than those in suspensions. The suspended cells grew well on 100 mg l(-1) of phenol only at NaCl concentrations below 0.44 M. However, the cells in microporous hollow fibers could completely degrade 500 mg l(-1) of phenol in solutions containing NaCl concentration up to 1.52 M, which was due to the enhanced tolerance limit to salinity effect by the membrane-attached biofilms and the sufficiently slow mass transfer of NaCl through the membrane pores.
一种微孔聚丙烯（PP）中空纤维膜接触器被用作生物反应器，在30℃下由恶臭假单胞菌BCRC 14365降解水溶液中的苯酚。纤维用乙醇预湿以使其更具亲水性。初始细胞密度固定为0.025 g l(-1)。研究了底物溶液中添加的NaCl浓度（0 - 1.78 M）和pH（3 - 8）对生物降解的影响。讨论了悬浮细胞的实验结果。结果表明，与悬浮液中的细胞相比，微孔中空纤维中的细胞对底物溶液pH的耐受范围较小。仅在NaCl浓度低于0.44 M时，悬浮细胞才能在100 mg l(-1)的苯酚上良好生长。然而，微孔中空纤维中的细胞可以在NaCl浓度高达1.52 M的溶液中完全降解500 mg l(-1)的苯酚，这是由于膜附着生物膜对盐度效应的耐受极限提高，以及NaCl通过膜孔的传质足够缓慢。
