Langeveld M, Gamadia L E, ten Berge I J M
Laboratory for Experimental Immunology, University of Amsterdam, the Netherlands.
Eur J Clin Invest. 2006 Apr;36(4):250-6. doi: 10.1111/j.1365-2362.2006.01626.x.
When analyzing human cellular immune responses, most focus is placed on the peripheral blood (PB) and, to a lesser extent, the lymph nodes. To date the spleen has not been analyzed with regard to its role in adaptive cellular immunity and more notably not with respect to T-cell immune responses.
We analyzed the splenic lymphocyte compartment in comparison with the PB lymphocyte compartment regarding the number of NK cells, B cells, CD4(+), CD8(+) T cells and CMV-specific CD8(+) T cells. Furthermore, we analyzed the distribution of naive, memory and effector subsets of CD4(+) and CD8(+) T cells in these compartments.
The spleen contains proportionally more B cells and less CD4(+) and CD8(+) T cells than PB. The percentage of CD8(+) T cells is greater in the spleen, leading to an inverse CD4/CD8 ratio. Both splenic CD4(+) and CD8(+) T-cell populations show a greater number of activated cells, and splenic CD8(+) T cells show a more differentiated cytotoxic CD27(-)CD45RA(+) memory phenotype.
Our findings show that the distribution of the different lymphocyte subsets is markedly different between the spleen and the PB, thus inferring an important and distinct role for the spleen in CD4(+) and CD8(+) T-cell activation.
在分析人类细胞免疫反应时，大多数研究集中在外周血（PB），对淋巴结的研究较少。迄今为止，尚未对脾脏在适应性细胞免疫中的作用进行分析，更具体地说，尚未研究其在T细胞免疫反应中的作用。
我们比较分析了脾脏淋巴细胞区室和外周血淋巴细胞区室中的自然杀伤细胞（NK细胞）、B细胞、CD4(+)、CD8(+) T细胞以及巨细胞病毒特异性CD8(+) T细胞的数量。此外，我们还分析了这些区室中CD4(+)和CD8(+) T细胞的初始、记忆和效应亚群的分布情况。
与外周血相比，脾脏中B细胞比例更高，而CD4(+)和CD8(+) T细胞比例更低。脾脏中CD8(+) T细胞的百分比更高，导致CD4/CD8比值倒置。脾脏中的CD4(+)和CD8(+) T细胞群体均显示出更多的活化细胞，并且脾脏中的CD8(+) T细胞表现出更分化的细胞毒性CD27(-)CD45RA(+)记忆表型。
我们的研究结果表明，脾脏和外周血中不同淋巴细胞亚群的分布存在显著差异，从而推断出脾脏在CD4(+)和CD8(+) T细胞活化中具有重要且独特的作用。
