Weinberger Birgit, Lazuardi Lutfan, Weiskirchner Ilka, Keller Michael, Neuner Christoph, Fischer Karl-Heinz, Neuman Ber, Würzner Reinhard, Grubeck-Loebenstein Beatrix
Immunology Division, Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria.
Hum Immunol. 2007 Feb;68(2):86-90. doi: 10.1016/j.humimm.2006.10.019. Epub 2006 Dec 5.
Despite general acceptance that immunologic changes are associated with aging and latent infection with Cytomegalovirus (CMV), no clear-cut distinction has so far been made between strictly age-related and CMV-induced changes. We therefore compared CD4+ and CD8+ naïve (CD45RA+CD28+), memory (CD45RA-CD28+), and effector (CD28-) T cells in CMV-positive (n = 164) and CMV-negative (n = 87) elderly persons and correlated CD8+ and CD4+ effector T cells with other T-cell subpopulations. Percentages of CD8+ as well as CD4+ effector T cells were higher, but percentages of naïve and memory cells were lower in CMV-positive compared to CMV-negative elderly persons. Negative correlations within CD8+ T-cell subsets were found to be present in both CMV-positive and CMV-negative elderly individuals. In contrast, correlations within CD4+ T-cell subpopulations and a positive correlation between CD8+ and CD4+ effector T cells were found in CMV-positive individuals only. Our results demonstrate that (a) in the elderly different T-cell subsets compete for space within the CD8+, but not the CD4+ T-cell population; (b) CMV induces changes in the CD4+ compartment that differ from the solely age-related changes seen in CMV-negative elderly population; and (c) the CMV-status of a population has to be taken into account before a conclusion on the effect of aging on the composition of the T-cell pool can be reached.
尽管人们普遍认为免疫变化与衰老以及巨细胞病毒（CMV）潜伏感染有关，但迄今为止，严格的年龄相关变化和CMV诱导的变化之间尚未明确区分。因此，我们比较了CMV阳性（n = 164）和CMV阴性（n = 87）老年人的CD4 +和CD8 +初始（CD45RA + CD28 +）、记忆（CD45RA - CD28 +）和效应（CD28 -）T细胞，并将CD8 +和CD4 +效应T细胞与其他T细胞亚群进行关联分析。与CMV阴性老年人相比，CMV阳性老年人中CD8 +以及CD4 +效应T细胞的百分比更高，但初始和记忆细胞的百分比更低。在CMV阳性和CMV阴性老年个体中均发现CD8 + T细胞亚群内存在负相关。相比之下，仅在CMV阳性个体中发现CD4 + T细胞亚群内存在相关性以及CD8 +和CD4 +效应T细胞之间存在正相关。我们的结果表明：（a）在老年人中，不同的T细胞亚群在CD8 + T细胞群体中竞争空间，但在CD4 + T细胞群体中并非如此；（b）CMV诱导CD4 +区室发生变化，这些变化不同于CMV阴性老年人群中仅与年龄相关的变化；（c）在得出衰老对T细胞库组成影响的结论之前，必须考虑人群的CMV状态。
