在人肝癌/痘苗病毒系统中表达的具有生物活性的重组凝血酶原和抗凝血酶III。

Biologically active recombinant prothrombin and antithrombin III expressed in a human hepatoma/vaccinia virus system.

作者信息

Russo G, Mertens K, De Magistris L, Lange H, Cortese R, Pietropaolo C

机构信息

Dipartimento di Biochimica e Biotecnologie Mediche, II Facoltà di Medicina e Chirurgia, Università di Napoli Federico II, Italy.

出版信息

Biotechnol Appl Biochem. 1991 Oct;14(2):222-33.

PMID:1662049

Abstract

Several systems have been devised that are based on viral promoters suitable for gene expression in eukaryotic cells. One such system, vaccinia virus, has been successfully used to express a variety of heterologous proteins following the construction of a recombinant virus. Indeed, because of the ability of vaccinia virus to infect a wide range of host cells, the expression system can be custom-designed so that a cell line can be instrumental in ensuring the correct processing of the recombinant polypeptide. We show that recombinant vaccinia virus and human hepatoma cells in culture are an efficient expression system with which to produce correctly modified and biologically active human prothrombin (15 micrograms/10(7) cells) and antithrombin III (40 micrograms/10(7) cells).

摘要

已经设计出了几种基于适用于真核细胞基因表达的病毒启动子的系统。其中一种这样的系统，即痘苗病毒，在构建重组病毒后已成功用于表达多种异源蛋白质。实际上，由于痘苗病毒能够感染多种宿主细胞，因此可以对表达系统进行定制设计，以便细胞系有助于确保重组多肽的正确加工。我们表明，培养中的重组痘苗病毒和人肝癌细胞是一种高效的表达系统，可用于产生正确修饰且具有生物活性的人凝血酶原（15微克/10^7个细胞）和抗凝血酶III（40微克/10^7个细胞）。