Puri S, Handa G, Kalsotra A K, Gupta V K, Shawl A S, Suri O P, Qazi G N
Regional Research Laboratory, Canal Road, Jammu-180001, India.
J Chromatogr Sci. 2006 Apr;44(4):177-80. doi: 10.1093/chromsci/44.4.177.
St. John's Wort (Hypericum perforatum), a perennial flowering plant, has been used medicinally for thousands of years and has most recently been identified as an effective treatment for mild to moderate depression and neuralgic disorders. This work presents a procedure for the isolation of naphthodianthrones from St. John's Wort by an accelerated extraction and separation of marker compounds by preparative high-performance liquid chromatography (HPLC) with photodiode array detection. The accelerated extraction method minimizes the extraction time and increases the yield, and the marker compounds obtained by preparative HPLC are of 98% purity. The compounds are characterized by liquid chromatography-mass spectrometry (electrospray ionization) and NMR spectra.
圣约翰草（贯叶连翘）是一种多年生开花植物，已被药用数千年，最近被确认为治疗轻度至中度抑郁症和神经紊乱的有效药物。这项工作提出了一种从圣约翰草中分离萘骈二蒽酮的方法，即通过加速提取，并利用配备光电二极管阵列检测的制备型高效液相色谱法（HPLC）对标记化合物进行分离。加速提取法可将提取时间减至最短并提高产量，通过制备型HPLC获得的标记化合物纯度为98%。这些化合物通过液相色谱 - 质谱联用（电喷雾电离）和核磁共振光谱进行表征。
