Tolonen Ari, Hohtola Anja, Jalonen Jorma
Department of Chemistry, University of Oulu, PO Box 3000, 90014 Oulu, Finland.
Phytochem Anal. 2003 Sep-Oct;14(5):306-9. doi: 10.1002/pca.720.
Hypericum perforatum L. (St. John's Wort) has been used in modern medicine for treatments of depression and neuralgic disorders. An HPLC method with photodiode array detection for the rapid determination of the major active compounds, naphthodianthrones and phloroglucinols, has been developed. The method permits the determination of hypericin, protohypericin, pseudohypericin, protopseudohypericin, hyperforin and adhyperforin in an extract in less than 5 min. Good linearity over the range 0.5-200 microg/mL for hyperforin and 0.02-100 microg/mL for hypericin was observed. Intra-assay accuracy and precision varied from 0.1 to 17% within these ranges. Lower levels of quantitative determination were 2 microg/mL for hyperforin and 0.5 microg/mL for hypericin, while detection limits were 0.1 and 0.02 microg/mL, respectively.
贯叶连翘已在现代医学中用于治疗抑郁症和神经紊乱。已开发出一种采用光电二极管阵列检测的高效液相色谱法，用于快速测定主要活性化合物萘并二蒽酮和间苯三酚。该方法能够在不到5分钟的时间内测定提取物中的金丝桃素、原金丝桃素、假金丝桃素、原假金丝桃素、金丝桃苷和去甲金丝桃苷。观察到金丝桃苷在0.5 - 200μg/mL范围内具有良好的线性，金丝桃素在0.02 - 100μg/mL范围内具有良好的线性。在这些范围内，批内准确度和精密度在0.1%至17%之间变化。金丝桃苷的定量测定下限为2μg/mL，金丝桃素的定量测定下限为0.5μg/mL，而检测限分别为0.1μg/mL和0.02μg/mL。
