Juenke JoEtta, Brown Paul I, Urry Francis M, McMillin Gwendolyn A
ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, Inc., 500 Chipeta Way, Salt Lake City, UT 84108, USA.
J Anal Toxicol. 2006 Jan-Feb;30(1):27-30. doi: 10.1093/jat/30.1.27.
This article describes a rapid isocratic high-performance liquid chromatographic (HPLC) method for the simultaneous measurement of the anticonvulsants levetiracetam and zonisamide. Monitoring these drugs is important for detecting potentially toxic concentrations, particularly in patients with renal impairment, but no commercial assays are currently available. Following a liquid-liquid extraction, levetiracetam (5-150 microg/mL) and zonisamide (5-80 microg/mL) are quantitated by HPLC-UV. The assay's limit of quantitation, linearity, imprecision, and accuracy adequately cover the therapeutic range of these drugs. The assay should be attractive to clinical laboratories because the run time for quantification of both drugs is approximately 5 min per sample, and no interferences are currently known.
本文介绍了一种快速等度高效液相色谱（HPLC）法，用于同时测定抗惊厥药左乙拉西坦和唑尼沙胺。监测这些药物对于检测潜在的中毒浓度很重要，尤其是在肾功能不全的患者中，但目前尚无商业化检测方法。经过液液萃取后，通过HPLC-UV对左乙拉西坦（5-150微克/毫升）和唑尼沙胺（5-80微克/毫升）进行定量。该检测方法的定量限、线性、不精密度和准确度足以覆盖这些药物的治疗范围。该检测方法对临床实验室应该具有吸引力，因为每个样品定量这两种药物的运行时间约为5分钟，且目前已知无干扰。
