P27kip1与细胞周期蛋白D3在非霍奇金淋巴瘤中的表达、定位及相互关系

[Expression, localization and interrelationship of P27kip1 and cyclin D3 in non-Hodgkin's lymphoma].

作者信息

Zhang Dong-mei, Lu Jian-xin, Shen Ai-guo, Chen Li, He Song, Shao Xiao-yi, Liu Hai-ou, Cheng Chun

机构信息

Department of Microbiology and Immunology, Nantong University, Nantong 226001, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2005 Dec;26(12):723-7.

PMID:16620575

Abstract

OBJECTIVE

To investigate the expression, localization and interrelationship of P27(kip1) and cyclin D3 in non-Hodgkin's lymphoma (NHL).

METHODS

The expressions of P27(kip1), cyclin D3 and index Ki-67 was detected in 100 NHL and 20 reactive lymph nodes by immunohistochemical technique. The expression and localization of P27(kip1) and cyclin D3 in 3 NHL cell lines were detected by Western blot, double immunolabelling and laser scanning confocal microscopy, respectively.

RESULTS

In general the expression of P27(kip1) in NHL was lower than in control group, and was negatively related to the tumor aggressiveness and proliferating activity; the expression of cyclin D3 in NHL was higher than in control group, and was positively related to the tumor aggressiveness and proliferating activity. There was a negative correlation between P27(kip1) and cyclin D3. Nevertheless, anomalous high P27(kip1) expression was found in a few NHL tissues with high expression of cyclin D3 and Ki-67. Overexpression and colocalization of P27(kip1) and cyclin D3 was found in Raji cell line.

CONCLUSIONS

Under expression of P27(kip1) and overexpression of cyclin D3 may play a role in the occurrence and development of NHL. Anomalous high P27(kip1) expression and its interaction with cyclin D3 may be another mechanism for tumor genesis of NHL.

摘要

目的

研究P27（kip1）和细胞周期蛋白D3在非霍奇金淋巴瘤（NHL）中的表达、定位及相互关系。

方法

采用免疫组织化学技术检测100例NHL和20例反应性淋巴结中P27（kip1）、细胞周期蛋白D3的表达及Ki-67指数。分别采用蛋白质免疫印迹法、双重免疫标记法及激光扫描共聚焦显微镜检测3种NHL细胞系中P27（kip1）和细胞周期蛋白D3的表达及定位。

结果

总体上，NHL中P27（kip1）的表达低于对照组，且与肿瘤侵袭性和增殖活性呈负相关；NHL中细胞周期蛋白D3的表达高于对照组，且与肿瘤侵袭性和增殖活性呈正相关。P27（kip1）与细胞周期蛋白D3之间呈负相关。然而，在少数细胞周期蛋白D3和Ki-67高表达的NHL组织中发现P27（kip1）异常高表达。在Raji细胞系中发现P27（kip1）与细胞周期蛋白D3的过表达及共定位。