92例非霍奇金淋巴瘤中Skp2与p27(kip1)的表达及相关性

[Expression and correlation of Skp2 and p27(kip1) in 92 cases of non-Hodgkin's lymphoma].

作者信息

Lu Jian-xin, Zhang Dong-mei, Shen Ai-guo, Wang Yu-chan, He Song, Shao Xiao-yi, Liu Hai-ou, Cheng Chun

机构信息

Department of Microbiology and Immunology, Nantong University, 226001, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2007 May;29(5):373-7.

PMID:17892135

Abstract

OBJECTIVE

To investigate the expression and correlation of Skp2 and p27kipl in non-Hodgkin's lymphoma.

METHODS

The expression of Skp2, p27(kip1) and Ki-67 (the proliferation index)were detected in sections of 92 cases of non-Hodgkin's lymphoma (NHL) and 14 cases of reactive lymph nodes by immunohistochemistry and histopathology. The expression of Skp2 and p27(kip1) in 4 NHL cell lines were detected by Western blot.

RESULTS

The expression of Skp2 in NHL cases were significantly higher than that in reactive lymph nodes (except the germinal centers), positively correlated with proliferation activity, and an increasing tumor aggressiveness was associated with the increased expression of Skp2. The expression of p27(kip1) protein in NHL cases were significantly lower than that in reactive lymph nodes (except the germinal centers), negatively correlated with proliferation activity, and an increasing tumor aggressiveness was associated with decreased expression of p27(kip1). The statistical analysis indicated that there was no obvious correlation between Skp2 and p27(kip1) expression in NHL tissues.

CONCLUSION

The higher expression of Skp2 and lower expression of p27(kip1) in NHL tissues may play a role in the tumorigenesis and development of NHL.

摘要

目的

探讨Skp2和p27kipl在非霍奇金淋巴瘤中的表达及相关性。

方法

采用免疫组织化学和组织病理学方法，检测92例非霍奇金淋巴瘤（NHL）组织和14例反应性淋巴结组织中Skp2、p27（kip1）及Ki-67（增殖指数）的表达。采用蛋白质印迹法检测4种NHL细胞系中Skp2和p27（kip1）的表达。

结果

NHL组织中Skp2的表达明显高于反应性淋巴结（生发中心除外），与增殖活性呈正相关，且肿瘤侵袭性增加与Skp2表达增加相关。NHL组织中p27（kip1）蛋白的表达明显低于反应性淋巴结（生发中心除外），与增殖活性呈负相关，且肿瘤侵袭性增加与p27（kip1）表达降低相关。统计学分析表明，NHL组织中Skp2与p27（kip1）的表达无明显相关性。