Jin Fang, Duan Yin-zhong, Li Xiao
Department of Orthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Jan;41(1):12-4.
To explore the effect and practical value of repairing the alveolar defects with tissue engineering technique and investigate the influence of the generated new bone on the orthodontic tooth movement.
The marrow stromal cells of rats were separated, cultured in vitro and induced to osteoblast-like cells. The osteoblast-like cells were implanted onto the ceramic bovine bone. Then the complex was implanted into the alveolar defect in one side of the rat's mandible. The other side of the mandible served as control. Eight weeks later, the orthodontic appliances were placed between the first molar and incisors of SD rats to move the first molar forward. The tooth movement and root resorption of the molar were observed.
We found that the tooth movement in the experimental area was faster than that in the normal alveolar bone (P < 0.05). The root resorption and the alveolar bone height loss were less than that in the control area (P < 0.05).
The tissue-engineered bone did not have negative influences on tooth movement. The repair of alveolar bone defect by tissue engineering approach may be used in craniofacial surgery and orthodontics.
探讨应用组织工程技术修复牙槽骨缺损的效果及实用价值，并研究新生骨对正畸牙齿移动的影响。
分离大鼠骨髓基质细胞，体外培养并诱导为成骨样细胞。将成骨样细胞接种于牛陶瓷骨上，然后将复合物植入大鼠下颌骨一侧的牙槽骨缺损处，下颌骨另一侧作为对照。8周后，在SD大鼠的第一磨牙与切牙之间放置正畸矫治器，使第一磨牙向前移动，观察磨牙的牙齿移动及牙根吸收情况。
发现实验区牙齿移动速度快于正常牙槽骨区（P < 0.05），牙根吸收及牙槽骨高度降低程度小于对照区（P < 0.05）。
组织工程骨对牙齿移动无负面影响。组织工程方法修复牙槽骨缺损可应用于颅颌面外科及正畸治疗。
