Li Yi-Han, Zhang Fei-Fei, Bao Shi-Jie, Wei Bin, Gong Yao
Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center of Stomatology, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology. Shanghai 200011, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2018 Oct;27(5):461-466.
To explore periodontal responses on the compression side during early tooth movement into alveolar defect regenerated by bone marrow mesenchymal stem cells (BMSCs) and porous granulated beta-tricalcium phosphate（β-TCP） scaffolds.
Thirty New Zealand rabbits were used to establish bilateral mandibular defective alveolar bone model by extracting the mandibular first molars and expanding the sockets. The right mandibular alveolar defects were filled with a construct of β-TCP scaffolds combined with BMSCs as experimental group. The left alveolar defects were repaired by β-TCP scaffolds alone as control group. Eight weeks later, 6 rabbits were sacrificed to evaluate osteogenesis effect. The other rabbits were loaded orthodontic force to move the bilateral second molars forward for 4 weeks. Six rabbits in each group were sacrificed at 1, 2, 3, and 4 week after orthodontic tooth movement (OTM). The distance of OTM was measured, and the status of periodontal tissues was observed by H-E staining. The number of osteoclasts on the compression side of tooth was counted by tartrate-resistant acid phosphatase histochemistry. The results were compared between groups using SPSS 19.0 software package.
After 8 weeks of bone grafting, the osteogenesis effect of the experimental group was better than the control group. The OTM distance in the experimental area was higher than that in the control area. At 2, 3 and 4 week of OTM, the number of osteoclasts in the experimental group was significantly higher than that in the control group.
A tissue-engineered complex with β-TCP scaffolds and BMSCs could well repair the alveolar bone defect. When the adjacent tooth moved into regenerated area, the new periodontal tissue had an active response, promoting to accelerate tooth movement.
探讨在骨髓间充质干细胞（BMSCs）和多孔颗粒状β-磷酸三钙（β-TCP）支架再生的牙槽骨缺损中，早期牙齿移动时压缩侧的牙周反应。
选用30只新西兰兔，通过拔除下颌第一磨牙并扩大牙槽窝建立双侧下颌骨缺损牙槽骨模型。右侧下颌牙槽骨缺损填充β-TCP支架与BMSCs构建物作为实验组。左侧牙槽骨缺损仅用β-TCP支架修复作为对照组。8周后，处死6只兔子评估成骨效果。其余兔子施加正畸力使双侧第二磨牙向前移动4周。在正畸牙齿移动（OTM）后1、2、3和4周，每组处死6只兔子。测量OTM距离，通过苏木精-伊红（H-E）染色观察牙周组织状态。用抗酒石酸酸性磷酸酶组织化学法计数牙齿压缩侧破骨细胞数量。使用SPSS 19.0软件包对组间结果进行比较。
植骨8周后，实验组成骨效果优于对照组。实验组区域的OTM距离高于对照组区域。在OTM后2、3和4周，实验组破骨细胞数量显著高于对照组。
β-TCP支架与BMSCs的组织工程复合物能很好地修复牙槽骨缺损。当相邻牙齿移入再生区域时，新的牙周组织有活跃反应，促进加速牙齿移动。
