Quinque Dominique, Kittler Ralf, Kayser Manfred, Stoneking Mark, Nasidze Ivan
Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D-04103, Leipzig, Germany.
Anal Biochem. 2006 Jun 15;353(2):272-7. doi: 10.1016/j.ab.2006.03.021. Epub 2006 Mar 30.
A simple noninvasive procedure for saliva sample collection and DNA extraction was developed. On average, the amount of human DNA (as measured by a TaqMan-based assay) was about 11.4 microg/mL saliva, which is more than can be obtained from other noninvasive samples such as cheek swabs. However, the presence of large amounts of nonhuman DNA (up to 90% of the total extracted DNA) in saliva samples does necessitate DNA quantitation methods that are specific for human DNA. We were able to reliably and accurately type different genetic markers (mDNA sequences, Y-chromosomal single-nucleotide polymorphisms, and autosomal microsatellite loci) from saliva samples stored for up to 30 days at 37 degrees C, making this method well-suited for field conditions and convenient transportation of samples back to the laboratory. Thus, saliva can be considered a reliable source of DNA for a wide variety of genetic studies.
开发了一种简单的非侵入性唾液样本采集和DNA提取程序。平均而言，人类DNA的量（通过基于TaqMan的检测方法测量）约为11.4微克/毫升唾液，这比从其他非侵入性样本（如口腔拭子）中获得的量更多。然而，唾液样本中存在大量非人类DNA（高达提取的总DNA的90%）确实需要针对人类DNA的定量方法。我们能够可靠且准确地对在37摄氏度下储存长达30天的唾液样本中的不同遗传标记（线粒体DNA序列、Y染色体单核苷酸多态性和常染色体微卫星位点）进行分型，这使得该方法非常适合现场条件以及将样本方便地运送回实验室。因此，唾液可被视为用于各种遗传研究的可靠DNA来源。
