Herráez David López, Stoneking Mark
Max Planck Institute for Evolutionary Anthropology, D-04103 Leipzig, Germany.
Anal Biochem. 2008 Dec 15;383(2):329-31. doi: 10.1016/j.ab.2008.08.015. Epub 2008 Aug 22.
Buccal cell samples are considered a reliable source of DNA for genotyping studies. However, a potential drawback is the presence of exogenous DNA that is coextracted with human genomic DNA. A set of saliva and cheek swab samples, in which the fraction of human DNA varies from 10 to 96%, was genotyped using the Affymetrix Mapping 500 K Array. Samples containing less than 30% human DNA performed poorly in terms of accuracy and reliability. Therefore, we recommend quantitating the amount of human DNA in buccal samples to be used for large-scale genotyping and eliminating samples with less than 30% human DNA.
颊细胞样本被认为是基因分型研究中可靠的DNA来源。然而，一个潜在的缺点是存在与人类基因组DNA共提取的外源DNA。使用Affymetrix Mapping 500 K芯片对一组唾液和口腔拭子样本进行基因分型，其中人类DNA的比例从10%到96%不等。人类DNA含量低于30%的样本在准确性和可靠性方面表现不佳。因此，我们建议对用于大规模基因分型的颊样本中的人类DNA量进行定量，并排除人类DNA含量低于30%的样本。
