Kim M R, Abd El-Aty A M, Kim I S, Shim J H
Natural Products Chemistry Laboratory, Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University, 300 Yong-Bong Dong, Buk-Ku, Gwangju 500-757, South Korea.
J Chromatogr A. 2006 May 26;1116(1-2):259-64. doi: 10.1016/j.chroma.2006.03.060. Epub 2006 Apr 18.
The rhizome of Angelica gigas Nakai, Angelica sinensis Diels and Angelica acutiloba Kitagawa (Umbelliferae) were chopped and extracted by hydrodistillation (HD) and solvent free solid injector (SFSI) methods to obtain the volatile compounds that were then characterized and identified by gas chromatography-mass spectrometry (GC/MS). SFSI extraction yielded a generally higher amount of volatile compounds than that produced by HD. A total of 48 components [including terpenes (8), aldehydes (4), alcohol (2), coumarins (9), Phthalide (3), acids (2) and sterols (2)] were identified by SFSI and gas chromatography-mass spectrometry from the three species of danggui cultivars, whereas, 24-essential oil was obtained by HD for Korean danggui only. According to these analyses, several coumarin derivatives such as decursinol angelate (16.83%) and decursin (29.34%) were found to be the dominant ones, followed by lomatin (10.25%) and marmesin (9.33%) in Korean danggui. Furfural and butylidene phthalide were the main components in Japanese in addition to butylidene dihydro-phthalide in Chinese danggui. Some parameters affecting the extraction efficiency such as injector temperature, injection time and pre-heating time were optimized. The results showed that the SFSI-method gave a higher yield of components with higher molecular mass than hydrodistillation. SFSI required little time to prepare the sample, little sample mass and a small quantity of organic solvent was needed. It can be concluded that analysis of volatile flavor compounds by SFSI in combination with gas chromatography/mass spectrometry is a suitable monitoring technique to differentiate danggui cultivars.
将当归（Angelica gigas Nakai）、当归（Angelica sinensis Diels）和东当归（Angelica acutiloba Kitagawa，伞形科）的根茎切碎，采用水蒸馏（HD）法和无溶剂固体进样器（SFSI）法进行提取，以获得挥发性化合物，然后通过气相色谱 - 质谱联用（GC/MS）对其进行表征和鉴定。SFSI提取法得到的挥发性化合物总量通常高于HD法。通过SFSI和气相色谱 - 质谱联用从三种当归品种中鉴定出总共48种成分[包括萜类（8种）、醛类（4种）、醇类（2种）、香豆素类（9种）、苯酞类（3种）、酸类（2种）和甾醇类（2种）]，而HD法仅从韩国当归中获得了24种精油。根据这些分析，发现几种香豆素衍生物，如当归酸正丁烯酯（16.83%）和紫花前胡醇（29.34%）是主要成分，其次是洛马亭（10.25%）和异补骨脂素（9.33%），存在于韩国当归中。除了丁烯基二氢苯酞存在于中国当归中外，糠醛和丁烯基苯酞是日本当归中的主要成分。对一些影响提取效率的参数，如进样器温度、进样时间和预热时间进行了优化。结果表明，SFSI法比水蒸馏法能获得更高产量的高分子量成分。SFSI法制备样品所需时间少、样品用量少且所需有机溶剂量少。可以得出结论，SFSI结合气相色谱/质谱联用分析挥发性风味化合物是区分当归品种的合适监测技术。
