Jalali Heravi M, Sereshti H
Department of Chemistry, Sharif University of Technology, P.O. Box 11365-9516, Tehran, Iran.
J Chromatogr A. 2007 Aug 10;1160(1-2):81-9. doi: 10.1016/j.chroma.2007.05.096. Epub 2007 Jun 7.
A novel method for extraction and analysis of volatile compounds of Artemisia haussknechtii Boiss., using simultaneous hydro-distillation and static headspace liquid microextraction followed by gas chromatography-mass spectrometry (SHD-SHLPME-GCMS) is developed. SHLPME parameters including nature of extracting solvent, headspace volume and design, extraction time, sample weight and microdrop volume were optimized. Comparison of hydro-distillation gas chromatography-mass spectrometry and HD-SHLPME-GCMS showed that the latter method is fast, simple, inexpensive and effective for the analysis of volatile compounds of aromatic plants. By using this method, 56 compounds were extracted and identified for Artemisia haussknechtii Boiss. The main constituents of its essential oil that were extracted by HD-SHLPME method, include camphor (41.01%), 1,8-cineole (32.35%), cis-davanone (3.68%), 4-terpineol (2.99%), linalool (2.84%), beta-fenchyl alcohol (2.72%), and borneol (2.58%).
开发了一种新方法，即采用同时蒸馏萃取和静态顶空液相微萃取结合气相色谱 - 质谱联用技术（SHD - SHLPME - GCMS）来提取和分析沙地蒿挥发性化合物。对SHLPME参数进行了优化，包括萃取溶剂的性质、顶空体积与设计、萃取时间、样品重量和微滴体积。水蒸馏气相色谱 - 质谱联用与HD - SHLPME - GCMS的比较表明，后一种方法对于分析芳香植物挥发性化合物快速、简便、廉价且有效。通过该方法，从沙地蒿中提取并鉴定出56种化合物。采用HD - SHLPME方法提取的其挥发油的主要成分包括樟脑（41.01%）、1,8 - 桉叶素（32.35%）、顺式 - 达瓦酮（3.68%）、4 - 松油醇（2.99%）、芳樟醇（2.84%）、β - 葑醇（2.72%）和冰片（2.58%）。
