Sortino Salvatore, Mazzaglia Antonino, Monsù Scolaro Luigi, Marino Merlo Francesca, Valveri Vincenza, Sciortino Maria Teresa
Dipartimento di Scienze Chimiche, Università di Catania,Viale Andrea Doria 8, I-95125 Catania, Italy.
Biomaterials. 2006 Aug;27(23):4256-65. doi: 10.1016/j.biomaterials.2006.03.035. Epub 2006 Apr 18.
The photodynamic activity of a carrier-sensitizer system consisting of heterotopic colloidal nanoparticles (diameter 100-1000 nm) of a cationic amphiphilic cyclodextrin, heptakis(2-omega-amino-O-oligo(ethylene oxide)-6-hexylthio)-beta-CD (SC6CDNH2) encapsulating the anionic 5,10,15,20-tetrakis(4-sulfonatophenyl)-21H,23H-porphyrin (TPPS) is investigated by an interdisciplinary approach involving the combination of time-resolved absorption and emission techniques with in vitro studies on cultured tumor cells. In a range of TPPS:SC6CDNH2 molar ratios between 1:10 and 1:50 these nanoparticles preserve the photodynamic properties of the entrapped photoactive agent. In fact, the triplet state of TPPS is efficiently populated, very long-lived and, as a consequence, able to produce singlet oxygen (the essential species for the photodynamic action) with quantum yield comparable to the free TPPS. Photodynamic efficacy of the carrier/sensitizer system is proven by in vitro studies on tumor Hela cells treated with TPPS:SC6CDNH2 at different molar ratio, showing significant cells death upon illumination with visible light.
通过将时间分辨吸收和发射技术与对培养肿瘤细胞的体外研究相结合的跨学科方法，研究了由阳离子两亲性环糊精七（2-ω-氨基-O-聚环氧乙烷-6-己硫基）-β-环糊精（SC6CDNH2）的异位胶体纳米颗粒（直径100 - 1000 nm）包裹阴离子型5,10,15,20-四（4-磺基苯基）-21H，23H-卟啉（TPPS）组成的载体-敏化剂系统的光动力活性。在TPPS:SC6CDNH2摩尔比为1:10至1:50的范围内，这些纳米颗粒保留了所包裹的光活性剂的光动力特性。事实上，TPPS的三重态被有效填充，寿命很长，因此能够产生单线态氧（光动力作用的关键物质），其量子产率与游离TPPS相当。通过对不同摩尔比的TPPS:SC6CDNH2处理的肿瘤海拉细胞进行体外研究，证明了载体/敏化剂系统的光动力功效，结果显示在可见光照射下细胞出现明显死亡。
