嗜肺军团菌的RpoS而非RelA在调节吞噬体生物发生及适应吞噬体微环境中的作用。
Role for RpoS but not RelA of Legionella pneumophila in modulation of phagosome biogenesis and adaptation to the phagosomal microenvironment.
作者信息
Abu-Zant Alaeddin, Asare Rexford, Graham James E, Abu Kwaik Yousef
机构信息
Department of Microbiology, University of Louisville College of Medicine, Louisville, KY 40292, USA.
出版信息
Infect Immun. 2006 May;74(5):3021-6. doi: 10.1128/IAI.74.5.3021-3026.2006.
Abstract
The induction of virulence traits by Legionella pneumophila at the post-exponential phase has been proposed to be triggered by the stringent response mediated by RelA, which triggers RpoS. We show that L. pneumophila rpoS but not relA is required for early intracellular survival and replication within human monocyte-derived macrophages and Acanthamoeba polyphaga. In addition, L. pneumophila rpoS but not relA is required for expression of the pore-forming activity. We provide evidence that RpoS plays a role in the modulation of phagosome biogenesis and in adaptation to the phagosomal microenvironment. Thus, there is no functional link between the stringent response and RpoS in the pathogenesis of L. pneumophila.
摘要
嗜肺军团菌在指数生长后期诱导毒力特性被认为是由RelA介导的严谨反应触发的，RelA会触发RpoS。我们发现，嗜肺军团菌的RpoS而非RelA是其在人单核细胞衍生巨噬细胞和多噬棘阿米巴中早期细胞内存活和复制所必需的。此外，嗜肺军团菌的RpoS而非RelA是形成孔道活性表达所必需的。我们提供的证据表明，RpoS在吞噬体生物发生的调节以及对吞噬体微环境的适应中发挥作用。因此，在嗜肺军团菌的发病机制中，严谨反应与RpoS之间不存在功能联系。
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