Hennig Ewa E, Allen Johnna M, Cover Timothy L
Department of Gastroenterology, Medical Center for Postgraduate Education, Cancer Center, Warsaw, Poland.
Infect Immun. 2006 May;74(5):3046-51. doi: 10.1128/IAI.74.5.3046-3051.2006.
Helicobacter pylori babA encodes an outer membrane protein that binds to fucosylated Lewis b blood group antigen. We analyzed a panel of 35 H. pylori strains and identified three possible chromosomal loci for babA. There was a significant association between the presence of babA and the presence of cagA (P = 0.0001). Phylogenetic analysis of babA alleles revealed two divergent families of signal sequences. Among 17 strains in which an intact in-frame babA allele was identified, 10 expressed a detectable BabA protein. Expression of a BabA protein and the Lewis b-binding phenotype were not dependent on the chromosomal locus of babA. These data indicate that there is marked heterogeneity among H. pylori strains in babA genetic content and BabA expression.
幽门螺杆菌babA编码一种与岩藻糖基化的Lewis b血型抗原结合的外膜蛋白。我们分析了一组35株幽门螺杆菌菌株，并确定了babA的三个可能的染色体位点。babA的存在与cagA的存在之间存在显著关联（P = 0.0001）。对babA等位基因的系统发育分析揭示了信号序列的两个不同家族。在鉴定出完整的读码框内babA等位基因的17株菌株中，10株表达了可检测到的BabA蛋白。BabA蛋白的表达和Lewis b结合表型不依赖于babA的染色体位点。这些数据表明，幽门螺杆菌菌株在babA基因含量和BabA表达方面存在显著异质性。
